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Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles
Joint Authors
Pugholm, Lotte Hatting
Revenfeld, Anne Louise Schacht
Søndergaard, Evo Kristina Lindersson
Jørgensen, Malene Møller
Bæk, Rikke
Varming, Kim
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Extracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system.
It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders.
Consequently, it is of great interest to investigate EVs in more detail with focus on immunological markers.
In this study, five major leukocyte subpopulations and the corresponding leukocyte-derived EVs were phenotyped with focus on selected immunological lineage-specific markers and selected vesicle-related markers.
The leukocyte-derived EVs displayed phenotypic differences in the 34 markers investigated.
The majority of the lineage-specific markers used for identification of the parent cell types could not be detected on EVs released from monocultures of the associated cell types.
In contrast, the vesicular presentation of CD9, CD63, and CD81 correlated to the cell surface expression of these markers, however, with few exceptions.
Furthermore, the cellular expression of CD9, CD63, and CD81 varied between leukocytes present in whole blood and cultured leukocytes.
In summary, these data demonstrate that the cellular and vesicular presentation of selected lineage-specific and vesicle-related markers may differ, supporting the accumulating observations that sorting of molecular cargo into EVs is tightly controlled.
American Psychological Association (APA)
Pugholm, Lotte Hatting& Bæk, Rikke& Søndergaard, Evo Kristina Lindersson& Revenfeld, Anne Louise Schacht& Jørgensen, Malene Møller& Varming, Kim. 2016. Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108843
Modern Language Association (MLA)
Pugholm, Lotte Hatting…[et al.]. Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles. Journal of Immunology Research No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1108843
American Medical Association (AMA)
Pugholm, Lotte Hatting& Bæk, Rikke& Søndergaard, Evo Kristina Lindersson& Revenfeld, Anne Louise Schacht& Jørgensen, Malene Møller& Varming, Kim. Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108843