Systemic Administration of Proteoglycan Protects BALBc Retired Breeder Mice from Experimental Arthritis
Joint Authors
Chiuso-Minicucci, Fernanda
Ishikawa, Larissa Lumi Watanabe
Colavite, Priscila Maria
Zorzella-Pezavento, Sofia Fernanda Gonçalves
Fraga-Silva, Thais F. C.
Mimura, Luiza A. N.
Marcolino, Larissa Doddi
Marques, Camila
França, Thais Graziela Donegá
Sartori, Alexandrina
Ikoma, Maura Rosane Valerio
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study was undertaken to evaluate the prophylactic potential of proteoglycan (PG) administration in experimental arthritis.
Female BALB/c retired breeder mice received two (2xPG50 and 2xPG100 groups) or three (3xPG50 group) intraperitoneal doses of bovine PG (50 μg or 100 μg) every three days.
A week later the animals were submitted to arthritis induction by immunization with three i.p.
doses of bovine PG associated with dimethyldioctadecylammonium bromide adjuvant at intervals of 21 days.
Disease severity was daily assessed after the third dose by score evaluation.
The 3xPG50 group showed significant reduction in prevalence and clinical scores.
This protective effect was associated with lower production of IFN-γ and IL-17 and increased production of IL-5 and IL-10 by spleen cells restimulated in vitro with PG.
Even though previous PG administration restrained dendritic cells maturation this procedure did not alter the frequency of regulatory Foxp3+ T cells.
Lower TNF-α and IL-6 levels and higher expression of ROR-γ and GATA-3 were detected in the paws of protected animals.
A delayed-type hypersensitivity reaction confirmed specific tolerance induction.
Taken together, these results indicate that previous PG inoculation determines a specific tolerogenic effect that is able to decrease severity of subsequently induced arthritis.
American Psychological Association (APA)
Ishikawa, Larissa Lumi Watanabe& Colavite, Priscila Maria& Fraga-Silva, Thais F. C.& Mimura, Luiza A. N.& França, Thais Graziela Donegá& Zorzella-Pezavento, Sofia Fernanda Gonçalves…[et al.]. 2016. Systemic Administration of Proteoglycan Protects BALBc Retired Breeder Mice from Experimental Arthritis. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108854
Modern Language Association (MLA)
Ishikawa, Larissa Lumi Watanabe…[et al.]. Systemic Administration of Proteoglycan Protects BALBc Retired Breeder Mice from Experimental Arthritis. Journal of Immunology Research No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1108854
American Medical Association (AMA)
Ishikawa, Larissa Lumi Watanabe& Colavite, Priscila Maria& Fraga-Silva, Thais F. C.& Mimura, Luiza A. N.& França, Thais Graziela Donegá& Zorzella-Pezavento, Sofia Fernanda Gonçalves…[et al.]. Systemic Administration of Proteoglycan Protects BALBc Retired Breeder Mice from Experimental Arthritis. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108854
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108854