Lactobacillus rhamnosus GG Activation of Dendritic Cells and Neutrophils Depends on the Dose and Time of Exposure
Joint Authors
Mahendran, Ratha
Cai, Shirong
Kandasamy, Matheswaran
Rahmat, Juwita N.
Tham, Sin Mun
Bay, Boon Huat
Lee, Yuan Kun
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study evaluates the ability of Lactobacillus rhamnosus GG (LGG) to activate DC and neutrophils and modulate T cell activation and the impact of bacterial dose on these responses.
Murine bone marrow derived DC or neutrophils were stimulated with LGG at ratios of 5 : 1, 10 : 1, and 100 : 1 (LGG : cells) and DC maturation (CD40, CD80, CD86, CD83, and MHC class II) and cytokine production (IL-10, TNF-α, and IL-12p70) were examined after 2 h and 18 h coculture and compared to the ability of BCG (the present immunotherapeutic agent for bladder cancer) to stimulate these cells.
A 2 h exposure to 100 : 1 (high dose) or an 18 h exposure to 5 : 1 or 10 : 1 (low dose), LGG : cells, induced the highest production of IL-12 and upregulation of CD40, CD80, CD86, and MHC II on DC.
In DCs stimulated with LGG activated neutrophils IL-12 production decreased with increasing dose.
LGG induced 10-fold greater IL-12 production than BCG.
T cell IFNγ and IL-2 production was significantly greater when stimulated with DC activated with low dose LGG.
In conclusion, DC or DC activated with neutrophils exposed to low dose LGG induced greater Th1 polarization in T cells and this could potentially exert stronger antitumor effects.
Thus the dose of LGG used for immunotherapy could determine treatment efficacy.
American Psychological Association (APA)
Cai, Shirong& Kandasamy, Matheswaran& Rahmat, Juwita N.& Tham, Sin Mun& Bay, Boon Huat& Lee, Yuan Kun…[et al.]. 2016. Lactobacillus rhamnosus GG Activation of Dendritic Cells and Neutrophils Depends on the Dose and Time of Exposure. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108870
Modern Language Association (MLA)
Cai, Shirong…[et al.]. Lactobacillus rhamnosus GG Activation of Dendritic Cells and Neutrophils Depends on the Dose and Time of Exposure. Journal of Immunology Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1108870
American Medical Association (AMA)
Cai, Shirong& Kandasamy, Matheswaran& Rahmat, Juwita N.& Tham, Sin Mun& Bay, Boon Huat& Lee, Yuan Kun…[et al.]. Lactobacillus rhamnosus GG Activation of Dendritic Cells and Neutrophils Depends on the Dose and Time of Exposure. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108870
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108870