CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata
Joint Authors
Yu, Mingjia
Yang, Shanjun
Sun, Hongxia
Xia, Qiang
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
As one of the surface membrane proteins of tetraspanin family, CD63 plays a crucial role in cellular trafficking and endocytosis, which also is associated with activation of a wide variety of immune cells.
Here, the homolog of CD63 was characterized from one marine mollusk, Paphia undulata, which is designated as Pu-CD63.
The complete cDNA of Pu-CD63 is 1,738 bp in length with an open reading frame (ORF) of 849 bp, encoding a 282 amino acid protein with four putative hydrophobic transmembrane helixes.
Bioinformatic analysis revealed that Pu-CD63 contains one putative YXXØ consensus motif of “110-YVII-113” and one N-glycosylation site “155-NGT-157” within the large extracellular loop (LEL) region, supporting its conserved function in plasma membrane and endosomal/lysosomal trafficking.
Moreover, temporal expression profile analysis demonstrates a drastic induction in the expression of CD63 in hemocytes after pathogenic challenge with either V.
parahaemolyticus or V.
alginolyticus.
By performing dsRNA-mediate RNAi knockdowns of CD63, a dramatic reduction in hemocytes phagocytic activity to pathogenic Vibrio is recorded by flow cytometry, revealing the definite role of Pu-CD63 in promoting hemocyte-mediated phagocytosis.
Therefore, our work has greatly enhanced our understanding about primitive character of innate immunity in marine mollusk.
American Psychological Association (APA)
Yu, Mingjia& Yang, Shanjun& Sun, Hongxia& Xia, Qiang. 2016. CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1108885
Modern Language Association (MLA)
Yu, Mingjia…[et al.]. CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata. Journal of Immunology Research No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1108885
American Medical Association (AMA)
Yu, Mingjia& Yang, Shanjun& Sun, Hongxia& Xia, Qiang. CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1108885
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108885