SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix
Joint Authors
Boysen, Marta
Schlicksupp, Laura
Dreher, Ingeborg
Loebbert, Ralf
Richter, Mario
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development.
Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics.
If biologics have Achilles’ heel, it is their potential immunogenicity.
To better understand the impact of the size of immune complexes of mAbs on anti-drug antibody (ADA) dependent adverse reactions in Macaca fascicularis, we developed an efficient high-throughput size exclusion chromatography- (SEC-) based methodology that enables analysis of the size, size distribution, and ratio of free and ADA-complexed mAb in serum allowing for assessment of formation and clearance of circulating ADA-mAb immune complexes (CIC).
American Psychological Association (APA)
Boysen, Marta& Schlicksupp, Laura& Dreher, Ingeborg& Loebbert, Ralf& Richter, Mario. 2016. SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108907
Modern Language Association (MLA)
Boysen, Marta…[et al.]. SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix. Journal of Immunology Research No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1108907
American Medical Association (AMA)
Boysen, Marta& Schlicksupp, Laura& Dreher, Ingeborg& Loebbert, Ralf& Richter, Mario. SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108907
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108907