Rotavirus Recombinant VP6 Nanotubes Act as an Immunomodulator and Delivery Vehicle for Norovirus Virus-Like Particles
Joint Authors
Malm, Maria
Tamminen, Kirsi
Lappalainen, Suvi
Vesikari, Timo
Blazevic, Vesna
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-04
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
We have recently shown that tubular form of rotavirus (RV) recombinant VP6 protein has an in vivo adjuvant effect on the immunogenicity of norovirus (NoV) virus-like particle (VLP) vaccine candidate.
In here, we investigated in vitro effect of VP6 on antigen presenting cell (APC) activation and maturation and whether VP6 facilitates NoV VLP uptake by these APCs.
Mouse macrophage cell line RAW 264.7 and dendritic cell line JAWSII were used as model APCs.
Internalization of VP6, cell surface expression of CD40, CD80, CD86, and major histocompatibility class II molecules, and cytokine and chemokine production were analyzed.
VP6 nanotubes were efficiently internalized by APCs.
VP6 upregulated the expression of cell surface activation and maturation molecules and induced secretion of several proinflammatory cytokines and chemokines.
The mechanism of VP6 action was shown to be partially dependent on lipid raft-mediated endocytic pathway as shown by methyl-β-cyclodextrin inhibition on tumor necrosis factor α secretion.
These findings add to the understanding of mechanism by which VP6 exerts its immunostimulatory and immunomodulatory actions and further support its use as a part of nonlive RV-NoV combination vaccine.
American Psychological Association (APA)
Malm, Maria& Tamminen, Kirsi& Lappalainen, Suvi& Vesikari, Timo& Blazevic, Vesna. 2016. Rotavirus Recombinant VP6 Nanotubes Act as an Immunomodulator and Delivery Vehicle for Norovirus Virus-Like Particles. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1108910
Modern Language Association (MLA)
Malm, Maria…[et al.]. Rotavirus Recombinant VP6 Nanotubes Act as an Immunomodulator and Delivery Vehicle for Norovirus Virus-Like Particles. Journal of Immunology Research No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1108910
American Medical Association (AMA)
Malm, Maria& Tamminen, Kirsi& Lappalainen, Suvi& Vesikari, Timo& Blazevic, Vesna. Rotavirus Recombinant VP6 Nanotubes Act as an Immunomodulator and Delivery Vehicle for Norovirus Virus-Like Particles. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1108910
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108910