Overcoming the Constraints of Anti-HIVCD89 Bispecific Antibodies That Limit Viral Inhibition
Joint Authors
Yu, Xiaocong
Duval, Mark
Gawron, Melissa
Posner, Marshall R.
Cavacini, Lisa A.
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-22
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Innovative strategies are necessary to maximize the clinical application of HIV neutralizing antibodies.
To this end, bispecific constructs of human antibody F240, reactive with well-conserved gp41 epitope and antibody 14A8, reactive with the IgA receptor (CD89) on effector cells, were constructed.
A F240 × 14A8 bispecific single chain variable region (scFv) molecule was constructed by linking two scFvs using a conventional GGGGS linker.
Despite immunoreactivity with HIV gp41 and neutrophils, this bispecific scFv failed to inhibit HIV infection.
This is in sharp contrast to viral inhibition using a chemical conjugate of the Fab of these two antibodies.
Therefore, we constructed two novel Fab-like bispecific antibody molecules centered on fusion of the IgG1 CH1 domain or CH1-hinge domain to the C-terminus of F240scFv and fusion of the kappa chain CL domain to the C-terminus of 14A8scFv.
Both Bi-Fab antibodies showed significant ADCVI activity for multiple clade B and clade C isolates by arming the neutrophils to inhibit HIV infection.
The approach presented in this study is unique for HIV immunotherapy in that the impetus of neutralization is to arm and mobilize PMN to destroy HIV and HIV infected cells.
American Psychological Association (APA)
Yu, Xiaocong& Duval, Mark& Gawron, Melissa& Posner, Marshall R.& Cavacini, Lisa A.. 2016. Overcoming the Constraints of Anti-HIVCD89 Bispecific Antibodies That Limit Viral Inhibition. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1108920
Modern Language Association (MLA)
Yu, Xiaocong…[et al.]. Overcoming the Constraints of Anti-HIVCD89 Bispecific Antibodies That Limit Viral Inhibition. Journal of Immunology Research No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1108920
American Medical Association (AMA)
Yu, Xiaocong& Duval, Mark& Gawron, Melissa& Posner, Marshall R.& Cavacini, Lisa A.. Overcoming the Constraints of Anti-HIVCD89 Bispecific Antibodies That Limit Viral Inhibition. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1108920
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108920