Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells
Joint Authors
Kameda, Takahiro
Yano, Kouji
Kubota, Tetsuo
Tozuka, Minoru
Ichimura, Naoya
Sato, Megumi
Yoshimoto, Akira
Ohkawa, Ryunosuke
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Apolipoprotein A-I (apoA-I), the main protein component of high-density lipoprotein (HDL), has many protective functions against atherosclerosis, one of them being cholesterol efflux capacity.
Although cholesterol efflux capacity measurement is suggested to be a key biomarker for evaluating the risk of development of atherosclerosis, the assay has not been optimized till date.
This study aims at investigating the effect of different states of cells on the cholesterol efflux capacity.
We also studied the effect of apoA-I modification by homocysteine, a risk factor for atherosclerosis, on cholesterol efflux capacity in different states of cells.
The cholesterol efflux capacity of apoA-I was greatly influenced by the extent of differentiation of THP-1 cells and attenuated by excessive foam cell formation.
N-Homocysteinylated apoA-I indicated a lower cholesterol efflux capacity than normal apoA-I in the optimized condition, whereas no significant difference was observed in the cholesterol efflux capacity between apoA-I in the excessive cell differentiation or foam cell formation states.
These results suggest that cholesterol efflux capacity of apoA-I varies depending on the state of cells.
Therefore, the cholesterol efflux assay should be performed using protocols optimized according to the objective of the experiment.
American Psychological Association (APA)
Yano, Kouji& Ohkawa, Ryunosuke& Sato, Megumi& Yoshimoto, Akira& Ichimura, Naoya& Kameda, Takahiro…[et al.]. 2016. Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells. Journal of Lipids،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108935
Modern Language Association (MLA)
Yano, Kouji…[et al.]. Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells. Journal of Lipids No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1108935
American Medical Association (AMA)
Yano, Kouji& Ohkawa, Ryunosuke& Sato, Megumi& Yoshimoto, Akira& Ichimura, Naoya& Kameda, Takahiro…[et al.]. Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells. Journal of Lipids. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108935
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108935