Extracellular Biofabrication, Characterization, and Antimicrobial Efficacy of Silver Nanoparticles Loaded on Cotton Fabrics Using Newly Isolated Streptomyces sp. SSHH-1E
Joint Authors
El-Naggar, Noura El-Ahmady
Mohamedin, Attiya
Hamza, Sarah Shawqi
Sherief, Abdel-Dayem
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-11
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Biological method for silver nanoparticles synthesis has been developed to obtain cost effective, clean, nontoxic, and ecofriendly size-controlled nanoparticles.
The objective of this study is extracellular biosynthesis of antimicrobial AgNPs using cell-free supernatant of a local Streptomyces sp.
strain SSHH-1E.
Different medium composition and fermentation conditions were screened for maximal AgNPs biosynthesis using Plackett-Burman experimental design and the variables with statistically significant effects were selected to study their combined effects and to find out the optimum values using a Box-Behnken design.
The synthesized AgNPs were characterized using UV-visible spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and energy dispersive X-ray spectroscopy.
Rapid biosynthesis of AgNPs was achieved by addition of 1 mM AgNO3 solution to the cell-free supernatant.
The produced particles showed a single surface plasmon resonance peak at 400 nm by UV-Vis spectroscopy which confirmed the presence of AgNPs.
Streptomyces sp.
SSHH-1E was identified as Streptomyces narbonensis SSHH-1E.
Transmission electron microscopy study indicated that the shape of AgNPs is spherical and the size is ranging from 20 to 40 nm.
Fourier transform infrared spectroscopy analysis provides evidence for proteins as possible reducing and capping agents.
Furthermore, the biosynthesized AgNPs significantly inhibited the growth of medically important pathogenic Gram-positive and Gram-negative bacteria and yeast.
The maximum biosynthesis of AgNPs was achieved at initial pH of 8, peptone of 0.5 g, and inoculum age of 48 h.
The statistical optimization resulted in a 4.5-fold increase in the production of AgNPs by Streptomyces narbonensis SSHH-1E.
American Psychological Association (APA)
El-Naggar, Noura El-Ahmady& Mohamedin, Attiya& Hamza, Sarah Shawqi& Sherief, Abdel-Dayem. 2016. Extracellular Biofabrication, Characterization, and Antimicrobial Efficacy of Silver Nanoparticles Loaded on Cotton Fabrics Using Newly Isolated Streptomyces sp. SSHH-1E. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1109104
Modern Language Association (MLA)
El-Naggar, Noura El-Ahmady…[et al.]. Extracellular Biofabrication, Characterization, and Antimicrobial Efficacy of Silver Nanoparticles Loaded on Cotton Fabrics Using Newly Isolated Streptomyces sp. SSHH-1E. Journal of Nanomaterials No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1109104
American Medical Association (AMA)
El-Naggar, Noura El-Ahmady& Mohamedin, Attiya& Hamza, Sarah Shawqi& Sherief, Abdel-Dayem. Extracellular Biofabrication, Characterization, and Antimicrobial Efficacy of Silver Nanoparticles Loaded on Cotton Fabrics Using Newly Isolated Streptomyces sp. SSHH-1E. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1109104
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109104