Nanoparticle-Mediated Photodynamic Therapy for Mixed Biofilms
Joint Authors
Usacheva, Marina
Layek, Buddhadev
Rahman Nirzhor, Saif S.
Prabha, Swayam
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Wounds associated with diabetes are difficult to heal and often stay unhealed, leading to higher morbidity and mortality in the diabetic population.
A major barrier to the successful healing of chronic diabetic wounds is the presence of biofilm-mediated infections.
In recent years, photodynamic therapy (PDT) has emerged as a promising treatment modality for the management of chronic wounds.
The objective of the present study was to evaluate the effectiveness of toluidine blue- (TB-) loaded nanoparticle-mediated PDT in eradicating antibiotic-resistant polymicrobial biofilms of Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus in an in vitro model.
Dioctyl sodium sulfosuccinate (aerosol OT, AOT)-alginate nanoparticles with high TB loading ( 10.8 ± 2.2 %) were formulated using a double emulsification cross-linking method.
TB nanoparticles induced effective killing of planktonic P.
aeruginosa (3.5 log10 CFU) and S.
aureus (>5 log10 CFU) and their combined biofilms (2.8 log10 CFU for P.
aeruginosa versus 3.4 log10 CFU for S.
aureus).
While P.
aeruginosa biofilm was more resistant when compared to that of S.
aureus, our results demonstrated effective eradication of complex biofilms of dual bacterial strains in vitro.
American Psychological Association (APA)
Usacheva, Marina& Layek, Buddhadev& Rahman Nirzhor, Saif S.& Prabha, Swayam. 2016. Nanoparticle-Mediated Photodynamic Therapy for Mixed Biofilms. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1109194
Modern Language Association (MLA)
Usacheva, Marina…[et al.]. Nanoparticle-Mediated Photodynamic Therapy for Mixed Biofilms. Journal of Nanomaterials No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1109194
American Medical Association (AMA)
Usacheva, Marina& Layek, Buddhadev& Rahman Nirzhor, Saif S.& Prabha, Swayam. Nanoparticle-Mediated Photodynamic Therapy for Mixed Biofilms. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1109194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109194