DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
Joint Authors
Reytblat, Irena
Lipovsky, Anat
Gedanken, Aharon
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Gene delivery is one of the steps necessary for gene therapy and for genetic modification.
However, delivering DNA into cells is challenging due to its negative charge that leads to repulsion by the negative cell membrane.
In the current research, DNA spheres with a DNA encoding to a certain gene were coated with bioavailable polymers, polyethylene imine (PEI) and polycaprolactone (PCL), in a short, one-step sonochemical reaction.
The polymers were used in order to neutralize the negative charge of the DNA.
Our study shows that the DNA nanospheres not only managed to penetrate the cell without causing it any damage, but also expressed the desired gene inside it.
American Psychological Association (APA)
Reytblat, Irena& Lipovsky, Anat& Gedanken, Aharon. 2016. DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109221
Modern Language Association (MLA)
Reytblat, Irena…[et al.]. DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts. Journal of Nanomaterials No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1109221
American Medical Association (AMA)
Reytblat, Irena& Lipovsky, Anat& Gedanken, Aharon. DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109221