Facile One-Pot Synthesis of Self-Assembled Folate-Biotin-Pullulan Nanoparticles for Targeted Intracellular Anticancer Drug Delivery
Joint Authors
Wang, Ming
Huang, Meiling
Wang, Jianting
Ye, Mingquan
Deng, Yan
Li, Honghao
Qian, Wensi
Zhu, Bolin
Zhang, Yao
Gong, Renmin
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The self-assembled folate-biotin-pullulan (FBP) nanoparticles (NPs) were prepared by facile one-pot synthesis and their physicochemical properties were characterized.
The self-assembled FBP NPs were used as an anticancer drug nanocarrier entrapping doxorubicin (DOX) for targeting folate-receptors-overexpressing cancer cells.
The identification of prepared NPs to folate-receptor-expressing cancer cells (KB cells) was affirmed by cell viability measurement, folate competition test, and flow cytometric analysis.
Compared with the naked DOX and DOX/BP NPs, the DOX/FBP NPs had lower IC50 value compared to KB cells as a result of the folate-receptor-mediated endocytosis process.
The cytotoxicity of DOX/FBP NPs to KB cells could be inhibited competitively by free folate.
The cellular intake pattern of naked DOX and drug-loaded NPs was identified by confocal laser scanning microscopy (CLSM) observation and the higher cellular uptake of drug for DOX/FBP NPs over naked DOX was observed.
The prepared FBP NPs had the potential to be used as a powerful carrier to target anticancer drugs to folate-receptor-expressing tumor cells and reduce cytotoxicity to normal tissues.
American Psychological Association (APA)
Wang, Ming& Huang, Meiling& Wang, Jianting& Ye, Mingquan& Deng, Yan& Li, Honghao…[et al.]. 2016. Facile One-Pot Synthesis of Self-Assembled Folate-Biotin-Pullulan Nanoparticles for Targeted Intracellular Anticancer Drug Delivery. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1109256
Modern Language Association (MLA)
Wang, Ming…[et al.]. Facile One-Pot Synthesis of Self-Assembled Folate-Biotin-Pullulan Nanoparticles for Targeted Intracellular Anticancer Drug Delivery. Journal of Nanomaterials No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1109256
American Medical Association (AMA)
Wang, Ming& Huang, Meiling& Wang, Jianting& Ye, Mingquan& Deng, Yan& Li, Honghao…[et al.]. Facile One-Pot Synthesis of Self-Assembled Folate-Biotin-Pullulan Nanoparticles for Targeted Intracellular Anticancer Drug Delivery. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1109256
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109256