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Enhancement of the Antibacterial Activity of Silver Nanoparticles against Phytopathogenic Bacterium Ralstonia solanacearum by Stabilization
Joint Authors
Chen, Juanni
Li, Shili
Luo, Jinxiang
Wang, Rongsheng
Ding, Wei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-01
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
In this paper, the enhanced antibacterial activity of silver nanoparticles (AgNPs) against the phytopathogenic bacterium Ralstonia solanacearum after stabilization using selected surfactants (SDS, SDBS, TX-100, and Tween 80) was examined, in comparison with silver ion.
Tween 80 was found to be the most preferable stabilizer of AgNPs due to the beneficial synergistic effects of the AgNPs and surfactant.
However, all the surfactants nearly had no effects on the antibacterial activity of Ag+.
In vitro, Tween 80-stabilized AgNPs showed the highest bactericidal activity against R.
solanacearum.
Further measurements using TEM, fluorescence microscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that though Ag+ and Tween 80-Ag+ induced high toxicity, Tween 80-stabilized AgNPs displayed most severe damage when in direct contact with cells, causing mechanistic injury to the cell membrane and strongly modifying and destructing the cellular proteins.
Meanwhile, in vivo, the pot experiments data indicated that the control efficiency of Tween 80-stabilized AgNPs on tobacco bacterial wilt was 96.71%, 90.11%, and 84.21%, at 7 days, 14 days, and 21 days, respectively.
Based on the results evidencing their advantageous low dosage requirements and strong antimicrobial activity, Tween 80-stabilized AgNPs are a promising antibacterial agent for use in alternative crop disease control approaches.
American Psychological Association (APA)
Chen, Juanni& Li, Shili& Luo, Jinxiang& Wang, Rongsheng& Ding, Wei. 2016. Enhancement of the Antibacterial Activity of Silver Nanoparticles against Phytopathogenic Bacterium Ralstonia solanacearum by Stabilization. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1109343
Modern Language Association (MLA)
Chen, Juanni…[et al.]. Enhancement of the Antibacterial Activity of Silver Nanoparticles against Phytopathogenic Bacterium Ralstonia solanacearum by Stabilization. Journal of Nanomaterials No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1109343
American Medical Association (AMA)
Chen, Juanni& Li, Shili& Luo, Jinxiang& Wang, Rongsheng& Ding, Wei. Enhancement of the Antibacterial Activity of Silver Nanoparticles against Phytopathogenic Bacterium Ralstonia solanacearum by Stabilization. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1109343
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109343