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Penetration and Silencing Activity of VEGF Dicer Substrate siRNA Vectorized by Chitosan Nanoparticles in Monolayer Culture and a Solid Tumor Model In Vitro for Potential Application in Tumor Therapy
Joint Authors
Abd Hamid, Zariyantey
Katas, Haliza
Raja, Maria Abdul Ghafoor
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-18
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Penetration and distribution of drug through the avascular regions of human solid tumors after extravasation are crucial concerns for antitumor efficacy.
To address this issue, an in vitro solid tumor model of multicellular layers (MCLs) of human colorectal cancer cells (DLD-1) was established.
In an attempt to deliver Dicer substrate small interfering RNA (DsiRNA), chitosan (CS) nanoparticles have been developed for targeting vascular endothelial growth factor (VEGF) gene for tumor growth inhibition.
The DsiRNA-CS nanoparticles prepared by ionic gelation method had provided maximal protection of DsiRNA in full human serum up to 48 h incubation.
RT-PCR studies revealed significant concentration- and time-dependent knock-down of VEGF mRNA and its product due to uniform penetration of DsiRNA-CS nanoparticles throughout MCLs.
Taken together, this study also demonstrated that DsiRNA-CS nanoparticles could effectively knock down VEGF gene as therapeutic target in monolayer culture or in solid tumor model for potential treatment of human colorectal carcinoma.
American Psychological Association (APA)
Raja, Maria Abdul Ghafoor& Katas, Haliza& Abd Hamid, Zariyantey. 2016. Penetration and Silencing Activity of VEGF Dicer Substrate siRNA Vectorized by Chitosan Nanoparticles in Monolayer Culture and a Solid Tumor Model In Vitro for Potential Application in Tumor Therapy. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1109346
Modern Language Association (MLA)
Raja, Maria Abdul Ghafoor…[et al.]. Penetration and Silencing Activity of VEGF Dicer Substrate siRNA Vectorized by Chitosan Nanoparticles in Monolayer Culture and a Solid Tumor Model In Vitro for Potential Application in Tumor Therapy. Journal of Nanomaterials No. 2016 (2016), pp.1-16.
https://search.emarefa.net/detail/BIM-1109346
American Medical Association (AMA)
Raja, Maria Abdul Ghafoor& Katas, Haliza& Abd Hamid, Zariyantey. Penetration and Silencing Activity of VEGF Dicer Substrate siRNA Vectorized by Chitosan Nanoparticles in Monolayer Culture and a Solid Tumor Model In Vitro for Potential Application in Tumor Therapy. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1109346
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109346