Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a PolyanilineGraphene Nanocomposite Functionalized Impedimetric Immunosensor
Joint Authors
Fu, JingJing
Shi, ZhuanZhuan
Li, Man
Wang, Yangyang
Yu, Ling
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The chondroitin sulphate proteoglycan 4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a tumor-associated antigen that is expressed in more than 85% of surgically removed melanoma lesions but has restricted distribution in normal tissues.
The diagnostic and therapeutic value of CSPG4 drives a need for sensitive and low-cost detection approaches.
To this end, we developed a polyaniline/graphene oxide nanocomposite (PANI@GO) that was electrochemically codeposited on indium tin oxide (ITO) electrode.
Glutaraldehyde mediated the covalent immobilization of CSPG4 specific antibody mAbD2.8.5 to construct a CSPG4 immunosensor using cell culture media and cell lysate as samples.
The fully assembled impedimetric immunosensor was used to detect CSPG4 in CSPG4-positive cell lines M14/CSPG4 and MV3.
No impedance signal changes could be observed from CSPG4-negative cell lines M14 and mAbMk2-23 showing the specificity of the CSPG4-impedimetric immunosensor.
This low-cost, simple, and label-free analytical method is an alternative to enzyme-linked immunosorbent assay and flow cytometry in screening of CSPG4 in complex biological samples.
American Psychological Association (APA)
Fu, JingJing& Shi, ZhuanZhuan& Li, Man& Wang, Yangyang& Yu, Ling. 2016. Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a PolyanilineGraphene Nanocomposite Functionalized Impedimetric Immunosensor. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109384
Modern Language Association (MLA)
Fu, JingJing…[et al.]. Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a PolyanilineGraphene Nanocomposite Functionalized Impedimetric Immunosensor. Journal of Nanomaterials No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1109384
American Medical Association (AMA)
Fu, JingJing& Shi, ZhuanZhuan& Li, Man& Wang, Yangyang& Yu, Ling. Label-Free Detection of Chondroitin Sulphate Proteoglycan 4 by a PolyanilineGraphene Nanocomposite Functionalized Impedimetric Immunosensor. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1109384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109384