A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting
Joint Authors
Can, Mehmet Erol
Çakmak, Hasan Basri
Dereli Can, Gamze
Ünverdi, Hatice
Toklu, Yasin
Hücemenoğlu, Sema
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Purpose.
To investigate the effect of platelet-rich fibrin (PRF) membrane on wound healing.
Methods.
Twenty-four right eyes of 24 New Zealand rabbits equally divided into 2 groups for the study design.
After the creation of 5 × 5 mm conjunctival damage, it was secured with PRF membrane, which was generated from the rabbit’s whole blood samples in PRF membrane group, whereas damage was left unsutured in the control group.
Three animals were sacrificed in each group on the 1st, 3rd, 7th, and 28th postoperative days.
Immunohistochemical (IHC) stainings and biomicroscopic evaluation were performed and compared between groups.
Results.
PRF membrane generated significant expressions of vascular endothelial growth factor (VEGF), transforming growth factor-beta (TGF- β ), and platelet-derived growth factor (PDGF) in the early postoperative period.
However, the IHC evaluation allowed showing the excessive staining at day 28, in control group.
Biomicroscopic evaluation revealed complete epithelialization in PRF membrane group, but none of the cases showed complete healing in the control group.
Conclusions.
This experimental study showed us the beneficial effects of the PRF membrane on conjunctival healing.
Besides its chemical effects, it provides mechanical support as a scaffold for the migrating cells that are important for ocular surface regeneration.
These overall results encourage us to apply autologous PRF membrane as a growth factor-enriched endogenous scaffold for ocular surface reconstruction.
American Psychological Association (APA)
Can, Mehmet Erol& Çakmak, Hasan Basri& Dereli Can, Gamze& Ünverdi, Hatice& Toklu, Yasin& Hücemenoğlu, Sema. 2016. A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1109877
Modern Language Association (MLA)
Can, Mehmet Erol…[et al.]. A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting. Journal of Ophthalmology No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1109877
American Medical Association (AMA)
Can, Mehmet Erol& Çakmak, Hasan Basri& Dereli Can, Gamze& Ünverdi, Hatice& Toklu, Yasin& Hücemenoğlu, Sema. A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1109877
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109877