Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers
Joint Authors
Zhou, Hongyan
Zhang, Wensong
Gao, Xunyi
Zhang, Hongguang
Kong, Ning
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-01
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Purpose.
The study aimed to evaluate the effect of all-trans retinoic acid-loaded nanostructured lipid carriers (ATRA-NLCs) on the zymosan-induced expression of the cytokines IL-4, IL-10, and IFN-γ and the matrix metalloproteinases/tissue inhibitor of metalloproteinases (MMPs/TIMPs) and TLR2 in rabbit corneal fibroblasts (RCFs).
Methods.
ATRA-NLCs were prepared by emulsification.
RCFs were isolated and harvested after four to seven passages in monolayer culture.
Cytokine release (IL-4, IL-10, and IFN-γ) induced by zymosan was analyzed by cytokine release assay, reverse transcription, and real-time polymerase chain reaction (RT-PCR) analysis detection.
MMP-1, MMP-3, and MMP-13, TIMP-1 and TIMP-2, and TLR2 expression were analyzed by immunoblotting.
Results.
ATRA-NLCs were resistant to light and physically stable, and the average size of the ATRA-NLCs was 200 nm.
ATRA-NLCs increased the zymosan-induced release of IL-4 and IL-10 and decreased the release of IFN-γ by RCFs.
ATRA-NLCs decreased the levels of TLR2 and MMPs/TIMPs above.
Conclusions.
ATRA may be a potent anti-inflammatory agent for the therapy of fungal keratitis (FK).
American Psychological Association (APA)
Zhou, Hongyan& Zhang, Wensong& Gao, Xunyi& Zhang, Hongguang& Kong, Ning. 2016. Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1109976
Modern Language Association (MLA)
Zhou, Hongyan…[et al.]. Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers. Journal of Ophthalmology No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1109976
American Medical Association (AMA)
Zhou, Hongyan& Zhang, Wensong& Gao, Xunyi& Zhang, Hongguang& Kong, Ning. Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1109976
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109976