Comparative Analysis of KnockOut™ Serum with Fetal Bovine Serum for the In Vitro Long-Term Culture of Human Limbal Epithelial Cells
Joint Authors
Zhang, Shaokun
Liu, Zaoxia
Su, Guanfang
Wu, Hong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The limbal epithelial cells can be maintained on 3T3 feeder layer with fetal bovine serum supplemented culture medium, and these cells have been used to successfully treat limbal stem cell deficiency.
However, fetal bovine serum contains unknown components and displays quantitative and qualitative lot-to-lot variations.
To improve the culture condition, the defined KnockOut serum replacement was investigated to replace fetal bovine serum for culturing human limbal epithelial cell.
Human primary limbal epithelial cells were cultured in KnockOut serum and fetal bovine serum supplemented medium, respectively.
The cell growth rate, gene expression, and maintenance of limbal epithelial stem cells were studied and compared between these two groups.
Human primary limbal epithelial cells were isolated and successfully serially cultivated in this novel KnockOut serum supplemented medium; the cell proliferation and stem cell maintenance were similar to those of cells grown in fetal bovine serum supplemented medium.
These data suggests that this KnockOut serum supplemented medium is an efficient replacement to traditional fetal bovine serum supplemented medium for limbal epithelial cell culture, and this medium has great potential for long term maintenance of limbal epithelial cells, limbal epithelial stem cells transplantation, and tissue regeneration.
American Psychological Association (APA)
Zhang, Shaokun& Liu, Zaoxia& Su, Guanfang& Wu, Hong. 2016. Comparative Analysis of KnockOut™ Serum with Fetal Bovine Serum for the In Vitro Long-Term Culture of Human Limbal Epithelial Cells. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1110058
Modern Language Association (MLA)
Zhang, Shaokun…[et al.]. Comparative Analysis of KnockOut™ Serum with Fetal Bovine Serum for the In Vitro Long-Term Culture of Human Limbal Epithelial Cells. Journal of Ophthalmology No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1110058
American Medical Association (AMA)
Zhang, Shaokun& Liu, Zaoxia& Su, Guanfang& Wu, Hong. Comparative Analysis of KnockOut™ Serum with Fetal Bovine Serum for the In Vitro Long-Term Culture of Human Limbal Epithelial Cells. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1110058
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110058