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Effects of Lutein on Hyperosmoticity-Induced Upregulation of IL-6 in Cultured Corneal Epithelial Cells and Its Relevant Signal Pathways
Joint Authors
Hu, Dan-Ning
Huang, Sheng-Chieh
Chao, Shih-Chun
Nien, Chan-Wei
Iacob, Codrin
Lin, Hung-Yu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Dry eye is a common disorder characterized by deficiency of tear.
Hyperosmoticity of tear stimulates inflammation and damage of ocular surface tissues and plays an essential role in the pathogenesis of dry eye.
Cultured human corneal epithelial (CE) cells were used for the study of effects of lutein and hyperosmoticity on the secretion of IL-6 by CE cells.
Cell viability of CE cells was not affected by lutein at 1–10 μM as determined by MTT assay.
Hyperosmoticity significantly elevated the secretion of IL-6 by CE cells as measured by ELISA analysis.
The constitutive secretion of IL-6 was not affected by lutein.
Lutein significantly and dose-dependently inhibited hyperosmoticity-induced secretion of IL-6.
Phosphorylated- (p)- p38 MAPK, p-JNK levels in cell lysates and NF-κB levels in cell nuclear extracts were increased by being exposed to hyperosmotic medium.
JNK, p38, and NF-κB inhibitors decreased hyperosmoticity-induced secretion of IL-6.
Lutein significantly inhibited hyperosmoticity-induced elevation of NF-κB, p38, and p-JNK levels.
We demonstrated that lutein inhibited hyperosmoticity-induced secretion of IL-6 in CE cells through the deactivation of p38, JNK, and NF-κB pathways.
Lutein may be a promising agent to be explored for the treatment of dry eye.
American Psychological Association (APA)
Chao, Shih-Chun& Nien, Chan-Wei& Iacob, Codrin& Hu, Dan-Ning& Huang, Sheng-Chieh& Lin, Hung-Yu. 2016. Effects of Lutein on Hyperosmoticity-Induced Upregulation of IL-6 in Cultured Corneal Epithelial Cells and Its Relevant Signal Pathways. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110089
Modern Language Association (MLA)
Chao, Shih-Chun…[et al.]. Effects of Lutein on Hyperosmoticity-Induced Upregulation of IL-6 in Cultured Corneal Epithelial Cells and Its Relevant Signal Pathways. Journal of Ophthalmology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1110089
American Medical Association (AMA)
Chao, Shih-Chun& Nien, Chan-Wei& Iacob, Codrin& Hu, Dan-Ning& Huang, Sheng-Chieh& Lin, Hung-Yu. Effects of Lutein on Hyperosmoticity-Induced Upregulation of IL-6 in Cultured Corneal Epithelial Cells and Its Relevant Signal Pathways. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110089
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110089