![](/images/graphics-bg.png)
Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress
Joint Authors
Sugimoto, Masahiko
Kondo, Mineo
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aim.
We investigated whether lecithin-bound iodine (LBI) can protect the integrity of tight junctions of retinal pigment epithelial cells from hypoxia.
Method.
Cultured human retinal pigment epithelial (ARPE-19) cells were pretreated with LBI.
To mimic hypoxic conditions, cells were incubated with CoCl2.
We compared the integrity of the tight junctions (TJs) of control to cells with either LBI alone, CoCl2 alone, or LBI + CoCl2.
The levels of cytokines in the conditioned media were also determined.
Results.
Significant decrease in the zonula occludens-1 (ZO-1) intensity in the CoCl2 group compared to the control (5787.7 ± 4126.4 in CoCl2 group versus 29244.6 ± 2981.2 in control; average ± standard deviation).
But the decrease was not significant in the LBI + CoCl2 (27189.0 ± 11231.1).
The levels of monocyte chemoattractant protein-1 (MCP-1) and Chemokine (C-C Motif) Ligand 11 (CCL-11) were significantly higher in the CoCl2 than in the control (340.8 ± 43.3 versus 279.7 ± 68.3 pg/mL for MCP-1, and 15.2 ± 12.9 versus 12.5 ± 6.1 pg/mL for CCL-11.
With LBI pretreatment, the levels of both cytokines were decreased to 182.6 ± 23.8 (MCP-1) and 5.46 ± 1.9 pg/mL for CCL-11).
Blockade of MCP-1 or CCL-11 also shows similar result representing TJ protection from hypoxic stress.
Conclusions.
LBI results in a protective action from hypoxia.
American Psychological Association (APA)
Sugimoto, Masahiko& Kondo, Mineo. 2016. Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress. Journal of Ophthalmology،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110119
Modern Language Association (MLA)
Sugimoto, Masahiko& Kondo, Mineo. Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress. Journal of Ophthalmology No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1110119
American Medical Association (AMA)
Sugimoto, Masahiko& Kondo, Mineo. Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress. Journal of Ophthalmology. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110119
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110119