Genotyping of Cryptosporidium Species and Their Clinical Manifestations in Patients with Renal Transplantation and Human Immunodeficiency Virus Infection
Joint Authors
Dey, Asmita
Ghoshal, Ujjala
Agarwal, Vikas
Ghoshal, Uday C.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In the present study we aimed to determine (i) frequency of Cryptosporidium species among patients with renal transplantation (RT) and human immunodeficiency virus (HIV) infection and (ii) relationship of the nature, severity, and duration of symptoms with different species and load of Cryptosporidium.
Stool samples from 70 (42 RT and 28 HIV) and 140 immunocompromised patients with and without cryptosporidiosis by modified Kinyoun’s staining were subjected to qPCR-melting curve analysis for identification of parasite species.
qPCR detected one microscopically negative sample to be positive for cryptosporidiosis.
C.
hominis, C.
parvum, and mixed infection were detected in 50/71 (70.4%), 19/71 (26.8%), and 2/71 (2.8%) patients, respectively.
Patients with cryptosporidiosis had higher stool frequency (median, IQR: 4, 3–6/d versus 3, 2–4/d; P = 0.017 ) and watery stool (52/71 [73%] versus 64/139 [46%]; P = 0.003 ).
Parasite load (median, IQR: Log10 6.37 (5.65–7.12), Log10 5.81 (4.26–6.65); P = 0.046 ) and nausea/vomiting (29/50 [58%] versus 5/19 [26%]; P = 0.032 ) were more frequent with C.
hominis than with C.
parvum infection.
Thus, Cryptosporidium spp.
(mainly C.
hominis) is a common cause of diarrhoea in RT and HIV patients.
American Psychological Association (APA)
Dey, Asmita& Ghoshal, Ujjala& Agarwal, Vikas& Ghoshal, Uday C.. 2016. Genotyping of Cryptosporidium Species and Their Clinical Manifestations in Patients with Renal Transplantation and Human Immunodeficiency Virus Infection. Journal of Pathogens،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1110202
Modern Language Association (MLA)
Dey, Asmita…[et al.]. Genotyping of Cryptosporidium Species and Their Clinical Manifestations in Patients with Renal Transplantation and Human Immunodeficiency Virus Infection. Journal of Pathogens No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1110202
American Medical Association (AMA)
Dey, Asmita& Ghoshal, Ujjala& Agarwal, Vikas& Ghoshal, Uday C.. Genotyping of Cryptosporidium Species and Their Clinical Manifestations in Patients with Renal Transplantation and Human Immunodeficiency Virus Infection. Journal of Pathogens. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1110202
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110202