![](/images/graphics-bg.png)
Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?
Joint Authors
de Godoy, Natalia Souza
Andrino, Marcos Luiz Alves
de Souza, Regina Maia
Gakiya, Erika
Amato, Valdir Sabbaga
Almeida Braz, Lucia Maria
Lindoso, José Angelo Lauletta
Source
Journal of Parasitology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL).
PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM.
Based on the GS, 38 samples from 32 patients were grouped: Group 1, 20 samples of VL cases, and Group 2, 18 samples of non-VL cases.
In order to evaluate the parasitological and molecular techniques in PB, the samples were examined.
From Group 1, PB kDNA-PCR was positive in 20 samples and in 19 of 20 in BM kDNA-PCR examination.
However, the parasitological examination of buffy coat was insensitive, being able to detect only 4 cases from Group 1.
All samples from Group 2 were negative.
We concluded that, for the diagnosis of visceral leishmaniasis, the parasitological examination of peripheral blood was not useful; however, molecular diagnosis by kDNA-PCR, performed in peripheral blood, could be useful to replace the parasitological examination of bone marrow.
American Psychological Association (APA)
de Godoy, Natalia Souza& Andrino, Marcos Luiz Alves& de Souza, Regina Maia& Gakiya, Erika& Amato, Valdir Sabbaga& Lindoso, José Angelo Lauletta…[et al.]. 2016. Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?. Journal of Parasitology Research،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110224
Modern Language Association (MLA)
de Godoy, Natalia Souza…[et al.]. Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?. Journal of Parasitology Research No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1110224
American Medical Association (AMA)
de Godoy, Natalia Souza& Andrino, Marcos Luiz Alves& de Souza, Regina Maia& Gakiya, Erika& Amato, Valdir Sabbaga& Lindoso, José Angelo Lauletta…[et al.]. Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?. Journal of Parasitology Research. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110224
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110224