Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis
Joint Authors
Ditgen, Dana
Brattig, Norbert
Hansmann, Jan
Winter, Dominic
Schramm, Guido
Erttmann, Klaus D.
Liebau, Eva
Anandarajah, Emmanuela M.
Source
Journal of Parasitology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-31
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense.
In the excretory/secretory (E/S) products of Strongyloides ratti and Trichuris suis sequences for thioredoxin (Trx) and Trx-like protein (Trx-lp) were identified.
To characterize the antioxidant Trx-lp and its interaction with the parasite’s mucosal habitat, S.
ratti and T.
suis Trx-lps were cloned and recombinantly expressed.
The primary antioxidative activity was assured by reduction of insulin and IgM.
Further analysis applying an in vitro mucosal 3D-cell culture model revealed that the secreted Trx-lps were able to bind to monocytic and intestinal epithelial cells and induce the time-dependent release of cytokines such as TNF-α, IL-22, and TSLP.
In addition, the redox proteins also possessed chemotactic activity for monocytic THP-1 cells and fostered epithelial wound healing activity.
These results confirm that the parasite-secreted Trx-lps are multifunctional proteins that can affect the host intestinal mucosa.
American Psychological Association (APA)
Ditgen, Dana& Anandarajah, Emmanuela M.& Hansmann, Jan& Winter, Dominic& Schramm, Guido& Erttmann, Klaus D.…[et al.]. 2016. Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis. Journal of Parasitology Research،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1110246
Modern Language Association (MLA)
Ditgen, Dana…[et al.]. Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis. Journal of Parasitology Research No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1110246
American Medical Association (AMA)
Ditgen, Dana& Anandarajah, Emmanuela M.& Hansmann, Jan& Winter, Dominic& Schramm, Guido& Erttmann, Klaus D.…[et al.]. Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis. Journal of Parasitology Research. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1110246
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110246