Bioluminescence-Sensing Assay for Microbial Growth Recognition

Abstract EN

The conventional methods for microbial viability quantification require cultivation and are laborious.

There is consequently a widespread need for cultivation-free methods.

The adenosine triphosphate (ATP) bioluminescence-sensing assay is considered an extremely effective biosensor; hence ATP is the energy currency of all living microbes and can be used as a rapid indicator of microbial viability.

We developed an ATP bioluminescence-sensing assay to detect microbial viability.

A bioluminescent recombinant E.

coli strain was used with luciferase extracted from transformed bacteria.

Results showed that there is a direct correlation between the bioluminescence intensity of the ATP bioluminescence-sensing assay and the microbial viability.

Bacterial counts from food samples were detected using the developed sensing assay and validated by the traditional plate-counting method.

Compared with the plate-counting method, ATP bioluminescence-sensing assay is a more rapid and efficient approach for detecting microbial viability.