Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models
Joint Authors
Liu, Wenjing
Sun, Zhaotian
Chen, Jinyu
Jing, Chuanbo
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Raman spectra of human colorectal tissue samples were employed to diagnose colorectal cancer.
High-quality Raman spectra were acquired from normal and cancerous colorectal tissues from 81 patients.
Subtle Raman variations, such as for peaks at 1134 cm−1 (protein, C-C/C-N stretching) and 1297 cm−1 (lipid, C-H2 twisting), were observed between normal and cancerous colorectal tissues.
The average peak intensity at 1134 and 1297 cm−1 was increased from approximately 235 and 72 in the normal group, respectively, to 315 and 273 in the cancer group.
The variations of Raman spectra reflected the changes of cell molecules during canceration.
The multivariate statistical methods of principal component analysis-linear discriminant analysis (PCA-LDA) and partial least-squares-discriminant analysis (PLS-DA), together with leave-one-patient-out cross-validation, were employed to build the discrimination model.
PCA-LDA was used to evaluate the capability of this approach for classifying colorectal cancer, resulting in a diagnostic accuracy of 79.2%.
Further PLS-DA modeling yielded a diagnostic accuracy of 84.3% for colorectal cancer detection.
Thus, the PLS-DA model is preferable between the two to discriminate cancerous from normal tissues.
Our results demonstrate that Raman spectroscopy can be used with an optimized multivariate data analysis model as a sensitive diagnostic alternative to identify pathological changes in the colon at the molecular level.
American Psychological Association (APA)
Liu, Wenjing& Sun, Zhaotian& Chen, Jinyu& Jing, Chuanbo. 2016. Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models. Journal of Spectroscopy،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1110758
Modern Language Association (MLA)
Liu, Wenjing…[et al.]. Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models. Journal of Spectroscopy No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1110758
American Medical Association (AMA)
Liu, Wenjing& Sun, Zhaotian& Chen, Jinyu& Jing, Chuanbo. Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models. Journal of Spectroscopy. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1110758
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110758