Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia
Joint Authors
Nakjarung, Kaewkanya
Bodhidatta, Ladaporn
Neesanant, Pimmnapar
Lertsethtakarn, Paphavee
Sethabutr, Orntipa
Vansith, Ket
Meng, Chhour Y.
Swierczewski, Brett E.
Mason, Carl J.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study investigated the genetic diversity of noroviruses identified from a previous surveillance study conducted at the National Pediatric Hospital in Phnom Penh, Cambodia, from 2004 to 2006.
In the previous study, 926 stool samples were collected from children aged 3–60 months with acute diarrhea (cases) and without diarrhea (controls) with reported 6.7% of cases and 3.2% of controls being positive for norovirus.
The initial norovirus diagnostic assay was performed with real-time reverse transcription-polymerase chain reaction (real-time RT PCR) which also distinguished between genogroups I and II (GI and GII).
Norovirus infection was most commonly detected in children aged 12–23 months in both cases and controls.
Norovirus Genotyping Tool and phylogenetic analysis of partial sequences of the 3′ end of the RNA-dependent RNA Polymerase (RdRp) and the capsid domain region were employed to assign genotypes of the norovirus strains.
GII.4 was the most predominant capsid genotype detected at 39.5% followed by GII.6 at 14.9%.
The GII.4 Hunter 2004 variant was the predominant strain detected.
Six RdRP/capsid recombinants including GII.P7/GII.6, GII.P7/GII.14, GII.P7/GII.20, GII.P12/GII.13, GII.P17/GII.16, and GII.P21/GII.3 were also identified.
This study of norovirus infection in young children in Cambodia suggests genetic diversity of norovirus as reported worldwide.
American Psychological Association (APA)
Nakjarung, Kaewkanya& Bodhidatta, Ladaporn& Neesanant, Pimmnapar& Lertsethtakarn, Paphavee& Sethabutr, Orntipa& Vansith, Ket…[et al.]. 2016. Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia. Journal of Tropical Medicine،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110845
Modern Language Association (MLA)
Nakjarung, Kaewkanya…[et al.]. Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia. Journal of Tropical Medicine No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1110845
American Medical Association (AMA)
Nakjarung, Kaewkanya& Bodhidatta, Ladaporn& Neesanant, Pimmnapar& Lertsethtakarn, Paphavee& Sethabutr, Orntipa& Vansith, Ket…[et al.]. Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia. Journal of Tropical Medicine. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1110845
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110845