FRTL-5 Rat Thyroid Cells Release Thyroglobulin Sequestered in Exosomes: A Possible Novel Mechanism for Thyroglobulin Processing in the Thyroid
Joint Authors
Vlasov, Pavel
Doi, Sonia Q.
Sellitti, Donald F.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Exosomes are 30–100 nm, membrane-bound vesicles containing specific cellular proteins, mRNAs, and microRNAs that take part in intercellular communication between cells.
A possible role for exosomes in thyroid function has not been fully explored.
In the present study, FRTL-5 rat thyroid cells were grown to confluence and received medium containing either thyroid stimulating hormone (TSH), exogenous bovine thyroglobulin (bTg), or neither additive for 24 or 48 hours followed by collection of spent medium and ultracentrifugation to isolate small vesicles.
Transmission electron microscopy and Western blotting for CD9 indicated the presence of exosomes.
Western blotting of exosome extract using a monoclonal anti-Tg antibody revealed a Tg-positive band at ~330 kDa (the expected size of monomeric Tg) with a higher density in TSH-treated cells compared to that in untreated cells.
These results are the first to show that normal thyroid cells in culture produce exosomes containing undegraded Tg.
American Psychological Association (APA)
Vlasov, Pavel& Doi, Sonia Q.& Sellitti, Donald F.. 2016. FRTL-5 Rat Thyroid Cells Release Thyroglobulin Sequestered in Exosomes: A Possible Novel Mechanism for Thyroglobulin Processing in the Thyroid. Journal of Thyroid Research،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110884
Modern Language Association (MLA)
Vlasov, Pavel…[et al.]. FRTL-5 Rat Thyroid Cells Release Thyroglobulin Sequestered in Exosomes: A Possible Novel Mechanism for Thyroglobulin Processing in the Thyroid. Journal of Thyroid Research No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1110884
American Medical Association (AMA)
Vlasov, Pavel& Doi, Sonia Q.& Sellitti, Donald F.. FRTL-5 Rat Thyroid Cells Release Thyroglobulin Sequestered in Exosomes: A Possible Novel Mechanism for Thyroglobulin Processing in the Thyroid. Journal of Thyroid Research. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1110884
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110884