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P-Selectin Targeted Dexamethasone-Loaded Lipid Nanoemulsions: A Novel Therapy to Reduce Vascular Inflammation
Joint Authors
Simion, Viorel
Constantinescu, Cristina Ana
Stan, Daniela
Deleanu, Mariana
Tucureanu, Monica Madalina
Butoi, Elena
Manduteanu, Ileana
Simionescu, Maya
Calin, Manuela
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-14
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Inflammation is a common process associated with numerous vascular pathologies.
We hypothesized that targeting the inflamed endothelium by coupling a peptide with high affinity for P-selectin to the surface of dexamethasone-loaded lipid nanoemulsions will highly increase their specific binding to activated endothelial cells (EC) and reduce the cell activation.
We developed and characterized dexamethasone-loaded lipid nanoemulsions directed towards P-selectin (PLN-Dex) and monitored their anti-inflammatory effects in vitro using cultured EC (EA.hy926 cells) and in vivo using a mouse model of acute inflammation [lipopolysaccharides (LPS) intravenously administered in C57BL/6 mice].
We found that PLN-Dex bound specifically to the surface of activated EC are efficiently internalized by EC and reduced the expression of proinflammatory genes, thus preventing the monocyte adhesion and transmigration to/through activated EC.
Given intravenously in mice with acute inflammation, PLN-Dex accumulated at a significant high level in the lungs (compared to nontargeted nanoemulsions) and significantly reduced mRNA expression level of key proinflammatory cytokines such as IL-1β, IL-6, and MCP-1.
In conclusion, the newly developed nanoformulation, PLN-Dex, is functional in vitro and in vivo, reducing selectively the endothelium activation and the consequent monocyte infiltration and diminishing significantly the lungs’ inflammation, in a mouse model of acute inflammation.
American Psychological Association (APA)
Simion, Viorel& Constantinescu, Cristina Ana& Stan, Daniela& Deleanu, Mariana& Tucureanu, Monica Madalina& Butoi, Elena…[et al.]. 2016. P-Selectin Targeted Dexamethasone-Loaded Lipid Nanoemulsions: A Novel Therapy to Reduce Vascular Inflammation. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1110938
Modern Language Association (MLA)
Simion, Viorel…[et al.]. P-Selectin Targeted Dexamethasone-Loaded Lipid Nanoemulsions: A Novel Therapy to Reduce Vascular Inflammation. Mediators of Inflammation No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1110938
American Medical Association (AMA)
Simion, Viorel& Constantinescu, Cristina Ana& Stan, Daniela& Deleanu, Mariana& Tucureanu, Monica Madalina& Butoi, Elena…[et al.]. P-Selectin Targeted Dexamethasone-Loaded Lipid Nanoemulsions: A Novel Therapy to Reduce Vascular Inflammation. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1110938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110938