Negative Impact of Hypoxia on Tryptophan 2,3-Dioxygenase Function
Joint Authors
Däubener, Walter
Elbers, Frank
Woite, Claudia
Antoni, Valentina
Stein, Sara
Funakoshi, Hiroshi
Nakamura, Toshikazu
Schares, Gereon
Eller, Silvia K.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Tryptophan is an essential amino acid for hosts and pathogens.
The liver enzyme tryptophan 2,3-dioxygenase (TDO) provokes, by its ability to degrade tryptophan to N-formylkynurenine, the precursor of the immune-relevant kynurenines, direct and indirect antimicrobial and immunoregulatory states.
Up to now these TDO-mediated broad-spectrum effector functions have never been observed under hypoxia in vitro, although physiologic oxygen concentrations in liver tissue are low, especially in case of infection.
Here we analysed recombinant expressed human TDO and ex vivo murine TDO functions under different oxygen conditions and show that TDO-induced restrictions of clinically relevant pathogens (bacteria, parasites) and of T cell proliferation are abrogated under hypoxic conditions.
We pinpointed the loss of TDO efficiency to the reduction of TDO activity, since cell survival and TDO protein levels were unaffected.
In conclusion, the potent antimicrobial as well as immunoregulatory effects of TDO were substantially impaired under hypoxic conditions that pathophysiologically occur in vivo.
This might be detrimental for the appropriate host immune response towards relevant pathogens.
American Psychological Association (APA)
Elbers, Frank& Woite, Claudia& Antoni, Valentina& Stein, Sara& Funakoshi, Hiroshi& Nakamura, Toshikazu…[et al.]. 2016. Negative Impact of Hypoxia on Tryptophan 2,3-Dioxygenase Function. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1110939
Modern Language Association (MLA)
Elbers, Frank…[et al.]. Negative Impact of Hypoxia on Tryptophan 2,3-Dioxygenase Function. Mediators of Inflammation No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1110939
American Medical Association (AMA)
Elbers, Frank& Woite, Claudia& Antoni, Valentina& Stein, Sara& Funakoshi, Hiroshi& Nakamura, Toshikazu…[et al.]. Negative Impact of Hypoxia on Tryptophan 2,3-Dioxygenase Function. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1110939
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110939