Reduced Numbers and Impaired Function of Regulatory T Cells in Peripheral Blood of Ischemic Stroke Patients
Joint Authors
Ruhnau, Johanna
Schulze, Juliane
von Sarnowski, Bettina
Heinrich, Marie
Langner, Sönke
Pötschke, Christian
Wilden, Anika
Kessler, Christof
Bröker, Barbara M.
Vogelgesang, Antje
Dressel, Alexander
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background and Purpose.
Regulatory T cells (Tregs) have been suggested to modulate stroke-induced immune responses.
However, analyses of Tregs in patients and in experimental stroke have yielded contradictory findings.
We performed the current study to assess the regulation and function of Tregs in peripheral blood of stroke patients.
Age dependent expression of CD39 on Tregs was quantified in mice and men.
Methods.
Total FoxP3+ Tregs and CD39+FoxP3+ Tregs were quantified by flow cytometry in controls and stroke patients on admission and on days 1, 3, 5, and 7 thereafter.
Treg function was assessed by quantifying the inhibition of activation-induced expression of CD69 and CD154 on T effector cells (Teffs).
Results.
Total Tregs accounted for 5.0% of CD4+ T cells in controls and <2.8% in stroke patients on admission.
They remained below control values until day 7.
CD39+ Tregs were most strongly reduced in stroke patients.
On day 3 the Treg-mediated inhibition of CD154 upregulation on CD4+ Teff was impaired in stroke patients.
CD39 expression on Treg increased with age in peripheral blood of mice and men.
Conclusion.
We demonstrate a loss of active FoxP3+CD39+ Tregs from stroke patient’s peripheral blood.
The suppressive Treg function of remaining Tregs is impaired after stroke.
American Psychological Association (APA)
Ruhnau, Johanna& Schulze, Juliane& von Sarnowski, Bettina& Heinrich, Marie& Langner, Sönke& Pötschke, Christian…[et al.]. 2016. Reduced Numbers and Impaired Function of Regulatory T Cells in Peripheral Blood of Ischemic Stroke Patients. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1110990
Modern Language Association (MLA)
Ruhnau, Johanna…[et al.]. Reduced Numbers and Impaired Function of Regulatory T Cells in Peripheral Blood of Ischemic Stroke Patients. Mediators of Inflammation No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1110990
American Medical Association (AMA)
Ruhnau, Johanna& Schulze, Juliane& von Sarnowski, Bettina& Heinrich, Marie& Langner, Sönke& Pötschke, Christian…[et al.]. Reduced Numbers and Impaired Function of Regulatory T Cells in Peripheral Blood of Ischemic Stroke Patients. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1110990
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110990