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New Role of Adult Lung c-kit+ Cells in a Mouse Model of Airway Hyperresponsiveness
Joint Authors
De Angelis, A.
Spaziano, Giuseppe
Matteis, Maria
D’Agostino, Bruno
Cappetta, Donato
Urbanek, Konrad
Piegari, Elena
Esposito, Grazia
Sgambato, Manuela
Tartaglione, Gioia
Russo, Rosa
De Palma, Raffaele
Rossi, Francesco
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Structural changes contribute to airway hyperresponsiveness and airflow obstruction in asthma.
Emerging evidence points to the involvement of c-kit+ cells in lung homeostasis, although their potential role in asthma is unknown.
Our aim was to isolate c-kit+ cells from normal mouse lungs and to test whether these cells can interfere with hallmarks of asthma in an animal model.
Adult mouse GFP-tagged c-kit+ cells, intratracheally delivered in the ovalbumin-induced airway hyperresponsiveness, positively affected airway remodeling and improved airway function.
In bronchoalveolar lavage fluid of cell-treated animals, a reduction in the number of inflammatory cells and in IL-4, IL-5, and IL-13 release, along with an increase of IL-10, was observed.
In MSC-treated mice, the macrophage polarization to M2-like subset may explain, at least in part, the increment in the level of anti-inflammatory cytokine IL-10.
After in vitro stimulation of c-kit+ cells with proinflammatory cytokines, the indoleamine 2,3-dioxygenase and TGFβ were upregulated.
These data, together with the increased apoptosis of inflammatory cells in vivo, indicate that c-kit+ cells downregulate immune response in asthma by influencing local environment, possibly by cell-to-cell contact combined to paracrine action.
In conclusion, intratracheally administered c-kit+ cells reduce inflammation, positively modulate airway remodeling, and improve function.
These data document previously unrecognized properties of c-kit+ cells, able to impede pathophysiological features of experimental airway hyperresponsiveness.
American Psychological Association (APA)
Spaziano, Giuseppe& Cappetta, Donato& Urbanek, Konrad& Piegari, Elena& Esposito, Grazia& Matteis, Maria…[et al.]. 2016. New Role of Adult Lung c-kit+ Cells in a Mouse Model of Airway Hyperresponsiveness. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1111041
Modern Language Association (MLA)
Spaziano, Giuseppe…[et al.]. New Role of Adult Lung c-kit+ Cells in a Mouse Model of Airway Hyperresponsiveness. Mediators of Inflammation No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1111041
American Medical Association (AMA)
Spaziano, Giuseppe& Cappetta, Donato& Urbanek, Konrad& Piegari, Elena& Esposito, Grazia& Matteis, Maria…[et al.]. New Role of Adult Lung c-kit+ Cells in a Mouse Model of Airway Hyperresponsiveness. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1111041
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111041