miR-146a Attenuates Inflammatory Pathways Mediated by TLR4NF-κB and TNFα to Protect Primary Human Retinal Microvascular Endothelial Cells Grown in High Glucose
Joint Authors
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Pathological mechanisms underlying diabetic retinopathy are still not completely understood.
Increased understanding of potential cellular pathways responsive to hyperglycemia is essential to develop novel therapeutic strategies for diabetic retinopathy.
A growing body of evidence shows that microRNA (miRNA) play important roles in pathological mechanisms involved in diabetic retinopathy, as well as possessing potential as novel therapeutic targets.
The hypothesis of this study was that miR-146a plays a key role in attenuating hyperglycemia-induced inflammatory pathways through reduced TLR4/NF-κB and TNFα signaling in primary human retinal microvascular endothelial cells (REC).
We cultured human REC in normal (5 mM) glucose or transferred to high glucose medium (25 mM) for 3 days.
Transfection was performed on REC with miRNA mimic (hsa-miR-146a-5p).
Our results demonstrate that miR-146a expression was decreased in human REC cultured in high glucose.
Overexpression of miR-146a using mimics reduced the levels of TLR4/NF-κB and TNFα in REC cultured in high glucose.
Both MyD88-dependent and -independent signaling were decreased by miR-146a overexpression in REC in high glucose conditions.
The results suggest that miR-146a is a potential therapeutic target for reducing inflammation in REC through inhibition of TLR4/NF-κB and TNFα.
Our study will contribute to understanding of diabetic retinal pathology, as well as providing important clues to develop therapeutics for clinical applications.
American Psychological Association (APA)
Ye, Eun-Ah& Steinle, Jena J.. 2016. miR-146a Attenuates Inflammatory Pathways Mediated by TLR4NF-κB and TNFα to Protect Primary Human Retinal Microvascular Endothelial Cells Grown in High Glucose. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111044
Modern Language Association (MLA)
Ye, Eun-Ah& Steinle, Jena J.. miR-146a Attenuates Inflammatory Pathways Mediated by TLR4NF-κB and TNFα to Protect Primary Human Retinal Microvascular Endothelial Cells Grown in High Glucose. Mediators of Inflammation No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1111044
American Medical Association (AMA)
Ye, Eun-Ah& Steinle, Jena J.. miR-146a Attenuates Inflammatory Pathways Mediated by TLR4NF-κB and TNFα to Protect Primary Human Retinal Microvascular Endothelial Cells Grown in High Glucose. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111044
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111044