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TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes
Joint Authors
Zheng, Hong
Xiao, Weidong
Qiu, Yuan
Pu, Aimin
Liu, Minqiang
Chen, Weigang
Wang, Wensheng
Yang, Hua
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
TLR2 signaling is related to colitis and involved in regulation of innate immunity in the intestinal tract, but the mechanisms remain unclear.
The aim of this study is to investigate how TLR2 affects differentiation of intraepithelial lymphocytes (IELs) and regulates the susceptibility of colitis.
IELs were isolated from the small intestine and colon of mice, respectively.
The IEL phenotype, activation, and apoptosis were examined using flow cytometry and RT-PCR.
IL-15 expression and IEL location were detected through immunohistochemistry.
The experimental colitis was induced by administration of dextran sulfate sodium (DSS).
We found that the numbers of CD8αα +, CD8αβ +, and TCRγδ + IELs were significantly decreased in TLR2-deficient mice and the residual IELs displayed reduced activation and proliferation and increased apoptosis, accompanied with impaired IL-15 expression by intestinal epithelial cells (IECs).
Further study showed that TLR2 signaling maintained the expression of IL-15 in IEC via NF-κB activation.
Moreover, TLR2-deficient mice were found to be more susceptible to DSS-induced colitis as shown by the increased severity of colitis.
Our results demonstrate that IECs contribute to the maintenance of IELs at least partly via TLR2-dependent IL-15 production, which provides a clue that may link IECs to innate immune protection of the host via IELs.
American Psychological Association (APA)
Qiu, Yuan& Pu, Aimin& Zheng, Hong& Liu, Minqiang& Chen, Weigang& Wang, Wensheng…[et al.]. 2016. TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1111060
Modern Language Association (MLA)
Qiu, Yuan…[et al.]. TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes. Mediators of Inflammation No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1111060
American Medical Association (AMA)
Qiu, Yuan& Pu, Aimin& Zheng, Hong& Liu, Minqiang& Chen, Weigang& Wang, Wensheng…[et al.]. TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1111060
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111060