Effects of Acute Endurance Exercise on Plasma Protein Profiles of Endurance-Trained and Untrained Individuals over Time

Abstract EN

Acute physical exercise and repeated exercise stimuli affect whole-body metabolic and immunologic homeostasis.

The aim of this study was to determine plasma protein profiles of trained (EET, n = 19 ) and untrained (SED, n = 17 ) individuals at rest and in response to an acute bout of endurance exercise.

Participants completed a bicycle exercise test at an intensity corresponding to 80% of their V O 2 m a x .

Plasma samples were taken before, directly after, and three hours after exercise and analyzed using multiplex immunoassays.

Seventy-eight plasma variables were included in the final analysis.

Twenty-nine variables displayed significant acute exercise effects in both groups.

Seven proteins differed between groups, without being affected by acute exercise.

Among these A2Macro and IL-5 were higher in EET individuals while leptin showed elevated levels in SED individuals.

Fifteen variables revealed group and time differences with elevated levels for IL-3, IL-7, IL-10, and TNFR2 in EET individuals.

An interaction effect could be observed for nine variables including IL-6, MMP-2, MMP-3, and muscle damage markers.

The proteins that differ between groups indicate a long-term exercise effect on plasma protein concentrations.

These findings might be of importance in the development of exercise-based strategies in the prevention and therapy of chronic metabolic and inflammatory diseases and for training monitoring.