NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation
Joint Authors
Zhou, Hong-Hao
Bao, Mei-hua
Li, Jian-Ming
Luo, Huai-qing
Tang, Liang
Lv, Qiao-li
Li, Guang-yi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
The present study was performed to investigate the effects and mechanisms of miR-99a on LPS-induced endothelial cell inflammation, as well as the regulation of NF-κB on miR-99a production.
Methods and Results.
ELISA showed that LPS treatment significantly promoted the secretion of inflammatory factors (TNF-α, IL-6, IL-1β, and MCP-1).
LPS treatment also inhibited miR-99a production and promoted mTOR expression and NF-κB nuclear translocation.
Overexpression of miR-99a suppressed the LPS-induced TNF-α, IL-6, IL-1β, and MCP-1 overproduction, mTOR upregulation, and NF-κB nuclear translocation.
The PROMO software analysis indicated NF-κB binding site in the −1643 to −1652 region of miR-99a promoter.
Dual luciferase reporter analysis, electrophoretic mobility shift assays (EMSA), and chromosome immunoprecipitation (ChIP) assays demonstrated that NF-κB promoted the transcription of miR-99a by binding to the −1643 to −1652 region of miR-99a promoter.
Further studies on HUVECs verified the regulatory effects of NF-κB on miR-99a production.
Conclusion.
MiR-99a inhibited the LPS-induced HUVECs inflammation via inhibition of the mTOR/NF-κB signal.
NF-κB promoted miR-99a production by binding to the −1643 to −1652 region of miR-99a promoter.
Considering the importance of endothelial inflammation on cardiovascular diseases, such as atherosclerosis, our results may provide a new insight into the pathogenesis and therapy of atherosclerosis.
American Psychological Association (APA)
Bao, Mei-hua& Li, Jian-Ming& Luo, Huai-qing& Tang, Liang& Lv, Qiao-li& Li, Guang-yi…[et al.]. 2016. NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111095
Modern Language Association (MLA)
Bao, Mei-hua…[et al.]. NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation. Mediators of Inflammation No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1111095
American Medical Association (AMA)
Bao, Mei-hua& Li, Jian-Ming& Luo, Huai-qing& Tang, Liang& Lv, Qiao-li& Li, Guang-yi…[et al.]. NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111095
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111095