The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice
Joint Authors
Leick, Edna Aparecida
Righetti, Renato F.
Martins-Olivera, Bruno Tadeu
Almeida-Reis, Rafael
Theodoro-Júnior, Osmar Aparecido
Oliva, Leandro Vilela
Neto dos Santos Nunes, Natalia
Olivo, Clarice Rosa
Vilela de Brito, Marlon
Prado, Carla Máximo
Oliva, Maria Luiza Vilela
Tibério, Iolanda F. L. C.
Martins, Milton Arruda
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-01
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD).
However, few resources for the inhibition of elastase have been investigated.
Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation.
Methods.
C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups).
At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining.
Results.
In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-α, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group.
rBbKI decreased the volume proportion of 8-iso-PGF2α, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group.
A reduction in the number of MUC-5-positive cells in the airway walls was also observed.
Conclusion.
rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling.
rBbKI may be a potential pharmacological tool for COPD treatment.
American Psychological Association (APA)
Martins-Olivera, Bruno Tadeu& Almeida-Reis, Rafael& Theodoro-Júnior, Osmar Aparecido& Oliva, Leandro Vilela& Neto dos Santos Nunes, Natalia& Olivo, Clarice Rosa…[et al.]. 2016. The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1111098
Modern Language Association (MLA)
Martins-Olivera, Bruno Tadeu…[et al.]. The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice. Mediators of Inflammation No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1111098
American Medical Association (AMA)
Martins-Olivera, Bruno Tadeu& Almeida-Reis, Rafael& Theodoro-Júnior, Osmar Aparecido& Oliva, Leandro Vilela& Neto dos Santos Nunes, Natalia& Olivo, Clarice Rosa…[et al.]. The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1111098
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111098