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Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon
Joint Authors
Chu, Fong-Fong
Gao, Lei
Gao, Qiang
Chen, Menglu
Chen, Pan
Feng, Dandan
Jiang, Yalin
Chang, Yongchao
Jin, Jianjun
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
5-HT enhances dextran sulfate sodium- (DSS-) induced colitis and is involved in inflammatory bowel disease (IBD).
Matrix metalloproteinases (MMPs) play roles in the process of intestinal inflammation.
Aims.
To examine whether 5-HT induces MMPs expression in mouse colon to enhance DSS-induced colitis.
Materials and Methods.
C57BL/6J (B6) mice were treated with either low-dose (1.0 mg/kg) or high-dose (2.0 mg/kg) 5-HT by enema, low-dose (1.0%) or high-dose (2.5%) DSS, or combined low-dose (1.0%) DSS and (1.0 mg/kg) 5-HT.
Mouse colitis was analyzed.
MMPs and tissue inhibitors of MMPs (TIMPs) mRNA were measured by real-time quantitative RT-PCR in mouse colon and in human Caco-2 cells and neutrophils.
MMP-3 and MMP-9 protein levels were quantified from immunohistochemistry (IHC) images of mouse colons.
Results.
5-HT exacerbated DSS-induced colitis, low-dose 5-HT induces both MMP-3 and MMP-9, and high-dose 5-HT only increased MMP-3 mRNA expression in mouse colon.
Mouse colon MMP-3 and MMP-9 protein levels were also elevated by 5-HT treatment.
The MMP-2, TIMP-1, and TIMP-2 mRNA levels were increased in the inflamed colon.
5-HT induced MMP-3 and MMP-9 mRNA expression in Caco-2 and human neutrophils, respectively, in vitro.
Conclusion.
5-HT induced MMP-3 and MMP-9 expression in mouse colon; these elevated MMPs may contribute to DSS-induced colitis.
American Psychological Association (APA)
Chen, Menglu& Gao, Lei& Chen, Pan& Feng, Dandan& Jiang, Yalin& Chang, Yongchao…[et al.]. 2016. Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111099
Modern Language Association (MLA)
Chen, Menglu…[et al.]. Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon. Mediators of Inflammation No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1111099
American Medical Association (AMA)
Chen, Menglu& Gao, Lei& Chen, Pan& Feng, Dandan& Jiang, Yalin& Chang, Yongchao…[et al.]. Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111099
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111099