Exaggerated IL-15 and Altered Expression of foxp3+ Cell-Derived Cytokines Contribute to Enhanced Colitis in Nlrp3−− Mice
Joint Authors
Beck, Paul L.
Ghosh, Subrata
Hirota, Simon A.
Ueno, Aito
Tulk, Sarah E.
Becker, Helen M.
Schenck, L. Patrick
Potentier, Mireille S.
Li, Yan
Muruve, Daniel A.
MacDonald, Justin A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The pathogenesis of Crohn’s disease (CD) involves defects in the innate immune system, impairing responses to microbes.
Studies have revealed that mutations NLRP3 are associated with CD.
We reported previously that Nlrp3−/− mice were more susceptible to colitis and exhibited reduced colonic IL-10 expression.
In the current study, we sought to determine how the loss of NLRP3 might be altering the function of regulatory T cells, a major source of IL-10.
Colitis was induced in wild-type (WT) and Nlrp3−/− mice by treatment with dextran sulphate sodium (DSS).
Lamina propria (LP) cells were assessed by flow cytometry and cytokine expression was assessed.
DSS-treated Nlrp3−/− mice exhibited increased numbers of colonic foxp3+ T cells that expressed significantly lower levels of IL-10 but increased IL-17.
This was associated with increased expression of colonic IL-15 and increased surface expression of IL-15 on LP dendritic cells.
Neutralizing IL-15 in Nlrp3−/− mice attenuated the severity of colitis, decreased the number of colonic foxp3+ cells, and reduced the colonic expression of IL-12p40 and IL-17.
These data suggest that the NLRP3 inflammasome can regulate intestinal inflammation through noncanonical mechanisms, providing additional insight as to how NLRP3 variants may contribute to the pathogenesis of CD.
American Psychological Association (APA)
Hirota, Simon A.& Ueno, Aito& Tulk, Sarah E.& Becker, Helen M.& Schenck, L. Patrick& Potentier, Mireille S.…[et al.]. 2016. Exaggerated IL-15 and Altered Expression of foxp3+ Cell-Derived Cytokines Contribute to Enhanced Colitis in Nlrp3−− Mice. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1111113
Modern Language Association (MLA)
Hirota, Simon A.…[et al.]. Exaggerated IL-15 and Altered Expression of foxp3+ Cell-Derived Cytokines Contribute to Enhanced Colitis in Nlrp3−− Mice. Mediators of Inflammation No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1111113
American Medical Association (AMA)
Hirota, Simon A.& Ueno, Aito& Tulk, Sarah E.& Becker, Helen M.& Schenck, L. Patrick& Potentier, Mireille S.…[et al.]. Exaggerated IL-15 and Altered Expression of foxp3+ Cell-Derived Cytokines Contribute to Enhanced Colitis in Nlrp3−− Mice. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1111113
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111113