NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-κB Signaling Pathway

Joint Authors

Huang, Jianhua
Li, Li
Yuan, Weifeng
Zheng, Linxin
Guo, Zhenhui
Huang, Wenjie

Source

Mediators of Inflammation

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-11-09

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Diseases

Abstract EN

The aim of the present study is to investigate the protective effects and relevant mechanisms exerted by NEMO-binding domain peptide (NBD) against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice.

The ALI model was induced by intratracheally administered atomized LPS (5 mg/kg) to BABL/c mice.

Half an hour before LPS administration, we treated the mice with increasing concentrations of intratracheally administered NBD or saline aerosol.

Two hours after LPS administration, each group of mice was sacrificed.

We observed that NBD pretreatment significantly attenuated LPS-induced lung histopathological injury in a dose-dependent manner.

Western blotting established that NBD pretreatment obviously attenuated LPS-induced IκB-α and NF-κBp65 activation and NOX1, NOX2, and NOX4 overexpression.

Furthermore, NBD pretreatment increased SOD and T-AOC activity and decreased MDA levels in lung tissue.

In addition, NBD also inhibited TNF-α and IL-1β secretion in BALF after LPS challenge.

In conclusion, NBD protects against LPS-induced ALI in mice.

American Psychological Association (APA)

Huang, Jianhua& Li, Li& Yuan, Weifeng& Zheng, Linxin& Guo, Zhenhui& Huang, Wenjie. 2016. NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-κB Signaling Pathway. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1111205

Modern Language Association (MLA)

Huang, Jianhua…[et al.]. NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-κB Signaling Pathway. Mediators of Inflammation No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1111205

American Medical Association (AMA)

Huang, Jianhua& Li, Li& Yuan, Weifeng& Zheng, Linxin& Guo, Zhenhui& Huang, Wenjie. NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-κB Signaling Pathway. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1111205

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1111205