Enterococcus faecium HDRsEf1 Protects the Intestinal Epithelium and Attenuates ETEC-Induced IL-8 Secretion in Enterocytes
Joint Authors
Tian, Zhongyuan
Liu, Xiaofang
Dai, Ran
Xiao, Yuncai
Wang, Xiliang
Bi, Dingren
Shi, Deshi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The probiotic Enterococcus faecium HDRsEf1 (Ef1) has been shown to have positive effects on piglet diarrhoea, but the mechanism has not yet been elucidated.
In this study, using the IPEC-J2 cell line to mimic intestinal epithelial cells and enterotoxigenic Escherichia coli (ETEC) K88ac as a representative intestinal pathogen, the mechanism underlying Ef1 protection against an enteropathogen was investigated.
The results demonstrated that Ef1 was effective in displacing K88ac from the IPEC-J2 cell layer.
Moreover, Ef1 and its cell-free supernatant (S-Ef1) modulate IL-8 released by IPEC-J2 cells.
Ef1 and its cell-free supernatant showed the potential to protect enterocytes from an acute inflammatory response.
In addition, Ef1 and its cell-free supernatant increased the transepithelial electrical resistance (TEER) of the enterocyte monolayer, thus strengthening the intestinal barrier against ETEC.
These results may contribute to the development of therapeutic interventions using Ef1 in intestinal disorders of piglets.
American Psychological Association (APA)
Tian, Zhongyuan& Liu, Xiaofang& Dai, Ran& Xiao, Yuncai& Wang, Xiliang& Bi, Dingren…[et al.]. 2016. Enterococcus faecium HDRsEf1 Protects the Intestinal Epithelium and Attenuates ETEC-Induced IL-8 Secretion in Enterocytes. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111213
Modern Language Association (MLA)
Tian, Zhongyuan…[et al.]. Enterococcus faecium HDRsEf1 Protects the Intestinal Epithelium and Attenuates ETEC-Induced IL-8 Secretion in Enterocytes. Mediators of Inflammation No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1111213
American Medical Association (AMA)
Tian, Zhongyuan& Liu, Xiaofang& Dai, Ran& Xiao, Yuncai& Wang, Xiliang& Bi, Dingren…[et al.]. Enterococcus faecium HDRsEf1 Protects the Intestinal Epithelium and Attenuates ETEC-Induced IL-8 Secretion in Enterocytes. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111213
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111213