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Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy
Joint Authors
Huang, Lvzhen
Li, Xiaoxin
Chen, Xiao-Li
Bai, Yu-Jing
Hu, Qin-Rui
Li, Shan-Shan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Epithelial-mesenchymal transition (EMT) of retinal pigment epithelium (RPE) is vital in proliferative vitreoretinopathy (PVR) development.
Apoptosis-stimulating proteins of p53 (ASPP2) have recently been reported to participate in EMT.
However, the role of ASPP2 in PVR pathogenesis has not been identified.
Methods.
Immunohistochemistry was used to investigate the expression of ASPP2 in epiretinal membranes of PVR patients.
ARPE-19 cells were transfected with ASPP2-siRNA, followed with measurement of cell cytotoxicity, proliferation, and migration ability.
EMT markers and related inflammatory and fibrosis cytokines were measured by western blot or flow cytometry.
Additionally, PVR rat models were induced by intravitreal injection of ARPE-19 cells transfected with ASPP2-siRNA and evaluated accordingly.
Results.
Immunofluorescence analysis revealed less intense expression of ASPP2 in PVR membranes.
ASPP2 knockdown facilitated the proliferation and migration of RPE cells and enhanced the expression of mesenchymal markers such as alpha smooth muscle actin, fibronectin, and ZEB1.
Meanwhile, ASPP2-siRNA increased EMT-related and inflammatory cytokines, including TGF- β , CTGF, VEGF, TNF-α, and interleukins.
PVR severities were more pronounced in the rat models with ASPP2-siRNA treatment.
Conclusions.
ASPP2 knockdown promoted EMT of ARPE-19 cells in vitro and exacerbated the progression of experimental PVR in vivo, possibly via inflammatory and fibrosis cytokines.
American Psychological Association (APA)
Chen, Xiao-Li& Bai, Yu-Jing& Hu, Qin-Rui& Li, Shan-Shan& Huang, Lvzhen& Li, Xiaoxin. 2016. Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1111224
Modern Language Association (MLA)
Chen, Xiao-Li…[et al.]. Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy. Mediators of Inflammation No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1111224
American Medical Association (AMA)
Chen, Xiao-Li& Bai, Yu-Jing& Hu, Qin-Rui& Li, Shan-Shan& Huang, Lvzhen& Li, Xiaoxin. Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1111224
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111224