Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages
Joint Authors
Rousseau, Alexandre
Pireaux, Valérie
Sauvage, Aude
Bihin, Benoît
Van Steenbrugge, Martine
Zouaoui Boudjeltia, Karim
Raes, Martine
Van Antwerpen, Pierre
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-30
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
Macrophages and oxidized LDLs play a key role in atherogenesis but their heterogeneity has been neglected up to now.
Macrophages are prone to polarization and subsets of polarized macrophages have been described in atheromas.
LDLs can be oxidized not only chemically by copper (Ox-LDLs) but also enzymatically by myeloperoxidase (MpOx-LDLs) resulting in oxidized LDLs poor in lipid peroxides.
The effects of physiologically relevant myeloperoxidase-oxidized LDLs on macrophage polarization or on polarized macrophages remain largely unknown.
In this study, the effects of LDLs on macrophage polarization were investigated by monitoring the expression of M1 and M2 genes following stimulation with native LDLs, Ox-LDLs, or MpOx-LDLs in RAW 264.7 cells.
Except for MRC1, which is induced only by Ox-LDLs, MpOx-LDLs induced an overexpression of most of the selected marker genes at the mRNA level.
MpOx-LDLs also modulate marker gene expression in polarized macrophages favoring notably anti-inflammatory Arg1 expression in M2 cells and also in the other phenotypes.
Noteworthy, MpOx-LDLs were the most efficient to accumulate lipids intracellularly in (un)polarized macrophages whatever the phenotype.
These data were largely confirmed in murine bone marrow-derived macrophages.
Our data suggest that MpOx-LDLs were the most efficient to accumulate within cells and to enhance an anti-inflammatory and antioxidant phenotype in M2 cells and also in the other macrophage phenotypes.
American Psychological Association (APA)
Pireaux, Valérie& Sauvage, Aude& Bihin, Benoît& Van Steenbrugge, Martine& Rousseau, Alexandre& Van Antwerpen, Pierre…[et al.]. 2016. Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-20.
https://search.emarefa.net/detail/BIM-1111249
Modern Language Association (MLA)
Pireaux, Valérie…[et al.]. Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages. Mediators of Inflammation No. 2016 (2016), pp.1-20.
https://search.emarefa.net/detail/BIM-1111249
American Medical Association (AMA)
Pireaux, Valérie& Sauvage, Aude& Bihin, Benoît& Van Steenbrugge, Martine& Rousseau, Alexandre& Van Antwerpen, Pierre…[et al.]. Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-20.
https://search.emarefa.net/detail/BIM-1111249
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111249