N-Acetyl-L-cysteine Protects the Enterocyte against Oxidative Damage by Modulation of Mitochondrial Function
Joint Authors
Yin, Yulong
Tan, Bi’e
Xiao, Hao
Wu, Miaomiao
Shao, Fangyuan
Guan, Guiping
Huang, Bo
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The neonatal small intestine is susceptible to damage caused by oxidative stress.
This study aimed to evaluate the protective role of antioxidant N-acetylcysteine (NAC) in intestinal epithelial cells against oxidative damage induced by H2O2.
IPEC-J2 cells were cultured in DMEM-H with NAC and H2O2.
After 2-day incubation, IPEC-J2 cells were collected for analysis of DNA synthesis, antioxidation capacity, mitochondrial respiration, and cell apoptosis.
The results showed that H2O2 significantly decreased (P<0.05) proliferation rate, mitochondrial respiration, and antioxidation capacity and increased cell apoptosis and the abundance of associated proteins, including cytochrome C, Bcl-XL, cleaved caspase-3, and total caspase-3.
NAC supplementation remarkably increased (P<0.05) proliferation rate, antioxidation capacity, and mitochondrial bioenergetics but decreased cell apoptosis.
These findings indicate that NAC might rescue the intestinal injury induced by H2O2.
American Psychological Association (APA)
Xiao, Hao& Wu, Miaomiao& Shao, Fangyuan& Guan, Guiping& Huang, Bo& Tan, Bi’e…[et al.]. 2016. N-Acetyl-L-cysteine Protects the Enterocyte against Oxidative Damage by Modulation of Mitochondrial Function. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111253
Modern Language Association (MLA)
Xiao, Hao…[et al.]. N-Acetyl-L-cysteine Protects the Enterocyte against Oxidative Damage by Modulation of Mitochondrial Function. Mediators of Inflammation No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1111253
American Medical Association (AMA)
Xiao, Hao& Wu, Miaomiao& Shao, Fangyuan& Guan, Guiping& Huang, Bo& Tan, Bi’e…[et al.]. N-Acetyl-L-cysteine Protects the Enterocyte against Oxidative Damage by Modulation of Mitochondrial Function. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111253
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111253