Dephosphorylation of Y685-VE-Cadherin Involved in Pulmonary Microvascular Endothelial Barrier Injury Induced by Angiotensin II
Joint Authors
Wu, Zhiyong
Wang, Zhiwei
Dai, Feifeng
Liu, Huagang
Ren, Wei
Chang, Jinxing
Li, Bowen
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Angiotensin II (AngII) caused pulmonary microvascular endothelial barrier injury, which induced acute aortic dissection (AAD) combined with acute lung injury (ALI).
However, the exact mechanism is unclear.
We investigated the role of dephosphorylation of Y685-VE-cadherin in the AngII induced pulmonary microvascular endothelial barrier injury.
Mice or pulmonary microvascular endothelial cells (PMVECs) were divided into control group, AngII group, AngII+PP2 (Src kinase inhibitor) group, and PP2 group.
PP2 was used to inhibit the phosphorylation of Y685-VE-cadherin.
Pathological changes, infiltration of macrophages and neutrophils, and pulmonary microvascular permeability were used to determine the pulmonary microvascular endothelial barrier function.
Flow cytometry was used to determine the apoptosis of PMVECs, and immunofluorescence was used to determine the skeletal arrangement.
Transendothelial resistance was used to detect the permeability of endothelial barrier.
Phosphorylation of Y685-VE-cadherin was significantly reduced after AngII stimulation (P<0.05), together with skeletal rearrangement, and elevation of endothelial permeability which finally induced endothelial barrier injury.
After PP2 interference, the phosphorylation of Y685-VE-cadherin was further reduced and the endothelial permeability was further elevated.
These data indicated that AngII could induce pulmonary injury by triggering endothelial barrier injury, and such process may be related to the dephosphorylation of Y685-VE-cadherin and the endothelial skeletal rearrangement.
American Psychological Association (APA)
Wu, Zhiyong& Wang, Zhiwei& Dai, Feifeng& Liu, Huagang& Ren, Wei& Chang, Jinxing…[et al.]. 2016. Dephosphorylation of Y685-VE-Cadherin Involved in Pulmonary Microvascular Endothelial Barrier Injury Induced by Angiotensin II. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111270
Modern Language Association (MLA)
Wu, Zhiyong…[et al.]. Dephosphorylation of Y685-VE-Cadherin Involved in Pulmonary Microvascular Endothelial Barrier Injury Induced by Angiotensin II. Mediators of Inflammation No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1111270
American Medical Association (AMA)
Wu, Zhiyong& Wang, Zhiwei& Dai, Feifeng& Liu, Huagang& Ren, Wei& Chang, Jinxing…[et al.]. Dephosphorylation of Y685-VE-Cadherin Involved in Pulmonary Microvascular Endothelial Barrier Injury Induced by Angiotensin II. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111270
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111270