MIF Promotes Classical Activation and Conversion of Inflammatory Ly6Chigh Monocytes into TipDCs during Murine Toxoplasmosis
Joint Authors
Robledo-Ávila, F.
de Dios Rosado, Juan
Satoskar, Abhay R.
Juárez, Imelda
Olguín, Jonadab E.
Vazquez-Mendoza, Alicia
Saavedra, Rafael
Partida-Sánchez, Santiago
Fernández, Jacquelina
Terrazas, Luis I.
Rodríguez-Sosa, Miriam
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-29
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Macrophage migration inhibitory factor (MIF) mediates immunity against Toxoplasma gondii infection by inducing inflammatory cytokines required to control the parasite replication.
However, the role of this inflammatory mediator in the cell-mediated immune response against this infection is still poorly understood.
Here, we used T.
gondii-infected WT and Mif−/− mice to analyze the role of MIF in the maturation of CD11b+ and CD8α+ dendritic cells (DCs).
We found that MIF promotes maturation of CD11b+ but not CD8α+ DCs, by inducing IL-12p70 production and CD86 expression.
Infected Mif−/− mice showed significantly lower numbers of TNF and inducible nitric oxide synthase- (iNOS-) producing DCs (TipDCs) compared to infected WT mice.
The adoptive transfer of Ly6Chigh monocytes into infected WT or Mif−/− mice demonstrated that MIF participates in the differentiation of Ly6Chigh monocytes into TipDCs.
In addition, infected Mif−/− mice display a lower percentage of IFN-γ-producing natural killer (NK) cells compared to WT mice, which is associated with reducing numbers of TipDCs in Mif−/− mice.
Furthermore, administration of recombinant MIF (rMIF) into T.
gondii-infected Mif−/− mice restored the numbers of TipDCs and reversed the susceptible phenotype of Mif−/− mice.
Collectively, these results demonstrate an important role for MIF inducing cell-mediated immunity to T.
gondii infection.
American Psychological Association (APA)
de Dios Rosado, Juan& Olguín, Jonadab E.& Juárez, Imelda& Saavedra, Rafael& Terrazas, Luis I.& Robledo-Ávila, F.…[et al.]. 2016. MIF Promotes Classical Activation and Conversion of Inflammatory Ly6Chigh Monocytes into TipDCs during Murine Toxoplasmosis. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1111283
Modern Language Association (MLA)
de Dios Rosado, Juan…[et al.]. MIF Promotes Classical Activation and Conversion of Inflammatory Ly6Chigh Monocytes into TipDCs during Murine Toxoplasmosis. Mediators of Inflammation No. 2016 (2016), pp.1-18.
https://search.emarefa.net/detail/BIM-1111283
American Medical Association (AMA)
de Dios Rosado, Juan& Olguín, Jonadab E.& Juárez, Imelda& Saavedra, Rafael& Terrazas, Luis I.& Robledo-Ávila, F.…[et al.]. MIF Promotes Classical Activation and Conversion of Inflammatory Ly6Chigh Monocytes into TipDCs during Murine Toxoplasmosis. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1111283
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111283