Uncoupling Protein 2 Increases Susceptibility to Lipopolysaccharide-Induced Acute Lung Injury in Mice
Joint Authors
Hu, Mingdong
Wang, Qin
Wang, Jianchun
Yang, Yu
Guo, Liang
Xu, Jing
Lei, Chuanjiang
Jiao, Yan
Xu, JianCheng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-07
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Uncoupling protein 2 (UCP2) is upregulated in patients with systemic inflammation and infection, but its functional role is unclear.
We up- or downregulated UCP2 expression using UCP2 recombinant adenovirus or the UCP2 inhibitor, genipin, in lungs of mice, and investigated the mechanisms of UCP2 in ALI.
UCP2 overexpression in mouse lungs increased LPS-induced pathological changes, lung permeability, lung inflammation, and lowered survival rates.
Furthermore, ATP levels and mitochondrial membrane potential were decreased, while reactive oxygen species production was increased.
Additionally, mitogen-activated protein kinases (MAPKs) activity was elevated, which increased the sensitivity to LPS-induced apoptosis and inflammation.
LPS-induced apoptosis and release of inflammatory factors were alleviated by pretreatment of the Jun N-terminal kinase (JNK) inhibitor SP600125 or the p38 MAPK inhibitor SB203580, but not by the extracellular signal-regulated kinase (ERK) inhibitor PD98059 in UCP2-overexpressing mice.
On the other hand, LPS-induced alveolar epithelial cell death and inflammation were attenuated by genipin.
In conclusion, UCP2 increased susceptibility to LPS-induced cell death and pulmonary inflammation, most likely via ATP depletion and activation of MAPK signaling following ALI in mice.
American Psychological Association (APA)
Wang, Qin& Wang, Jianchun& Hu, Mingdong& Yang, Yu& Guo, Liang& Xu, Jing…[et al.]. 2016. Uncoupling Protein 2 Increases Susceptibility to Lipopolysaccharide-Induced Acute Lung Injury in Mice. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1111286
Modern Language Association (MLA)
Wang, Qin…[et al.]. Uncoupling Protein 2 Increases Susceptibility to Lipopolysaccharide-Induced Acute Lung Injury in Mice. Mediators of Inflammation No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1111286
American Medical Association (AMA)
Wang, Qin& Wang, Jianchun& Hu, Mingdong& Yang, Yu& Guo, Liang& Xu, Jing…[et al.]. Uncoupling Protein 2 Increases Susceptibility to Lipopolysaccharide-Induced Acute Lung Injury in Mice. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1111286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111286