Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide
Joint Authors
Wu, Tianyou
Wang, Chao
Ding, Luoyang
Shen, Yizhao
Cui, Huihui
Wang, Mengzhi
Wang, Hongrong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
As one of functional active amino acids, L-arginine holds a key position in immunity.
However, the mechanism that arginine modulates cow mammary inflammatory response in ruminant is unclear.
Therefore, this study was conducted to investigate the effects of L-arginine on inflammatory response and casein expression after challenging the bovine mammary epithelial cells (BMECs) with lipopolysaccharide (LPS).
The cells were divided into four groups, stimulated with or without LPS (10 μg/mL) and treated with or without arginine (100 μg/mL) for 12 h.
The concentration of proinflammatory cytokines, inducible nitric oxide synthase (iNOS), mammalian target of rapamycin (mTOR), and Toll-like receptor 4 (TLR4) signaling pathways as well as the casein was determined.
The results showed that arginine reduced the LPS-induced production like IL-1β, IL-6, TNF-α, and iNOS.
Though the expression of NF-κB was attenuated and the mTOR signaling pathway was upregulated, arginine had no effect on TLR4 expression.
In addition, our results show that the content of β-casein and the total casein were enhanced after arginine was supplemented in LPS-induced BMECs.
In conclusion, arginine could relieve the inflammatory reaction induced by LPS and enhance the concentration of β-casein and the total casein in bovine mammary epithelial cells.
American Psychological Association (APA)
Wu, Tianyou& Wang, Chao& Ding, Luoyang& Shen, Yizhao& Cui, Huihui& Wang, Mengzhi…[et al.]. 2016. Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111320
Modern Language Association (MLA)
Wu, Tianyou…[et al.]. Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide. Mediators of Inflammation No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1111320
American Medical Association (AMA)
Wu, Tianyou& Wang, Chao& Ding, Luoyang& Shen, Yizhao& Cui, Huihui& Wang, Mengzhi…[et al.]. Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111320
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111320