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Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells
Joint Authors
Izakelian, Laura
Dubey, Anisha
Zhang, Grace
Wong, Steven
Kwofie, Karen
Qureshi, Aatif
Botelho, Fernando
Richards, Carl D.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
IL-33 modulates both innate and adaptive immune responses at tissue sites including lung and may play critical roles in inflammatory lung disease.
Although IL-33 expression can be altered upon NF-Kappa B activation, here we examine regulation by Oncostatin M, a gp130 cytokine family member, in mouse lung tissue.
Responses were assessed in BALB/c mouse lung at day 7 of transient overexpression using endotracheally administered adenovirus encoding OSM (AdOSM) or empty vector (AdDel70).
Whole lung extracts showed induction of IL-33 mRNA (>20-fold) and protein (10-fold increase in immunoblots) by AdOSM relative to AdDel70.
Immunohistochemistry for IL-33 indicated a marked induction of nuclear staining in alveolar epithelial cells in vivo.
Oncostatin M stimulated IL-33 mRNA and IL-33 full length protein in C10 mouse type 2 alveolar epithelial cells in culture in time-dependent and dose-dependent fashion, whereas IL-6, LIF, IL-31, IL-4, or IL-13 did not, and TGFβ repressed IL-33.
IL-33 induction was associated with activation of STAT3, and pharmacological inhibition of STAT3 ameliorated IL-33 levels.
These results indicate Oncostatin M as a potent inducer of IL-33 in mouse lung epithelial cells and suggest that an OSM/IL-33 axis may participate in innate immunity and inflammatory conditions in lung.
American Psychological Association (APA)
Richards, Carl D.& Izakelian, Laura& Dubey, Anisha& Zhang, Grace& Wong, Steven& Kwofie, Karen…[et al.]. 2016. Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1111335
Modern Language Association (MLA)
Richards, Carl D.…[et al.]. Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells. Mediators of Inflammation No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1111335
American Medical Association (AMA)
Richards, Carl D.& Izakelian, Laura& Dubey, Anisha& Zhang, Grace& Wong, Steven& Kwofie, Karen…[et al.]. Regulation of IL-33 by Oncostatin M in Mouse Lung Epithelial Cells. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1111335
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111335