Caffeine-Induced Suppression of GABAergic Inhibition and Calcium-Independent Metaplasticity
Author
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
GABAergic inhibition plays a critical role in the regulation of neuron excitability; thus, it is subject to modulations by many factors.
Recent evidence suggests the elevation of intracellular calcium ( [ Ca 2+ ] i ) and calcium-dependent signaling molecules underlie the modulations.
Caffeine induces a release of calcium from intracellular stores.
We tested whether caffeine modulated GABAergic transmission by increasing [ Ca 2+ ] i .
A brief local puff-application of caffeine to hippocampal CA1 pyramidal cells transiently suppressed GABAergic inhibitory postsynaptic currents (IPSCs) by 73.2 ± 6.98%.
Time course of suppression and the subsequent recovery of IPSCs resembled DSI (depolarization-induced suppression of inhibition), mediated by endogenous cannabinoids that require a [ Ca 2+ ] i rise.
However, unlike DSI, caffeine-induced suppression of IPSCs (CSI) persisted in the absence of a [ Ca 2+ ] i rise.
Intracellular applications of BAPTA and ryanodine (which blocks caffeine-induced calcium release from intracellular stores) failed to prevent the generation of CSI.
Surprisingly, ruthenium red, an inhibitor of multiple calcium permeable/release channels including those of stores, induced metaplasticity by amplifying the magnitude of CSI independently of calcium.
This metaplasticity was accompanied with the generation of a large inward current.
Although ionic basis of this inward current is undetermined, the present result demonstrates that caffeine has a robust Ca 2+ -independent inhibitory action on GABAergic inhibition and causes metaplasticity by opening plasma membrane channels.
American Psychological Association (APA)
Isokawa, Masako. 2016. Caffeine-Induced Suppression of GABAergic Inhibition and Calcium-Independent Metaplasticity. Neural Plasticity،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1112963
Modern Language Association (MLA)
Isokawa, Masako. Caffeine-Induced Suppression of GABAergic Inhibition and Calcium-Independent Metaplasticity. Neural Plasticity No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1112963
American Medical Association (AMA)
Isokawa, Masako. Caffeine-Induced Suppression of GABAergic Inhibition and Calcium-Independent Metaplasticity. Neural Plasticity. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1112963
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1112963