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Programming of Dopaminergic Neurons by Neonatal Sex Hormone Exposure: Effects on Dopamine Content and Tyrosine Hydroxylase Expression in Adult Male Rats
Joint Authors
Moya, Pablo R.
Espinosa, Pedro
Silva, Roxana A.
Sanguinetti, Nicole K.
Venegas, Francisca C.
Riquelme, Raul
González, Luis F.
Cruz, Gonzalo
Renard, Georgina M.
Sotomayor-Zárate, Ramón
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We sought to determine the long-term changes produced by neonatal sex hormone administration on the functioning of midbrain dopaminergic neurons in adult male rats.
Sprague-Dawley rats were injected subcutaneously at postnatal day 1 and were assigned to the following experimental groups: TP (testosterone propionate of 1.0 mg/50 μL); DHT (dihydrotestosterone of 1.0 mg/50 μL); EV (estradiol valerate of 0.1 mg/50 μL); and control (sesame oil of 50 μL).
At postnatal day 60, neurochemical studies were performed to determine dopamine content in substantia nigra-ventral tegmental area and dopamine release in nucleus accumbens.
Molecular (mRNA expression of tyrosine hydroxylase) and cellular (tyrosine hydroxylase immunoreactivity) studies were also performed.
We found increased dopamine content in substantia nigra-ventral tegmental area of TP and EV rats, in addition to increased dopamine release in nucleus accumbens.
However, neonatal exposure to DHT, a nonaromatizable androgen, did not affect midbrain dopaminergic neurons.
Correspondingly, compared to control rats, levels of tyrosine hydroxylase mRNA and protein were significantly increased in TP and EV rats but not in DHT rats, as determined by qPCR and immunohistochemistry, respectively.
Our results suggest an estrogenic mechanism involving increased tyrosine hydroxylase expression, either by direct estrogenic action or by aromatization of testosterone to estradiol in substantia nigra-ventral tegmental area.
American Psychological Association (APA)
Espinosa, Pedro& Silva, Roxana A.& Sanguinetti, Nicole K.& Venegas, Francisca C.& Riquelme, Raul& González, Luis F.…[et al.]. 2016. Programming of Dopaminergic Neurons by Neonatal Sex Hormone Exposure: Effects on Dopamine Content and Tyrosine Hydroxylase Expression in Adult Male Rats. Neural Plasticity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113148
Modern Language Association (MLA)
Espinosa, Pedro…[et al.]. Programming of Dopaminergic Neurons by Neonatal Sex Hormone Exposure: Effects on Dopamine Content and Tyrosine Hydroxylase Expression in Adult Male Rats. Neural Plasticity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113148
American Medical Association (AMA)
Espinosa, Pedro& Silva, Roxana A.& Sanguinetti, Nicole K.& Venegas, Francisca C.& Riquelme, Raul& González, Luis F.…[et al.]. Programming of Dopaminergic Neurons by Neonatal Sex Hormone Exposure: Effects on Dopamine Content and Tyrosine Hydroxylase Expression in Adult Male Rats. Neural Plasticity. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113148
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113148